Nanosilver Hydrogel Surface Raman Enhanced Scattering for Detection of Paraoxon

• 1、College of Chemistry and Chmical Engingeering,Hunan University,changsha 410082

Abstract：The uniform size surface enhanced raman scattering(SERS) substrate was prepared by photochemical, using one-pot method and without other reducing agents. Silver nanoparticles of about 200 nm were grown inside the hydrogel, realizing the idea of preparing a large-area silver-hydrogel SERS substrate. In order to apply the prepared SERS substrate as a biosensor, we further modified acetylcholinesterase on the hydrogel and successfully developed a new AChE method based on SERS to detect organic phosphorus. Acetylcholinesterase hydrolyzes thioacetylcholine to product the thiocholine with raman signal, and the presence of organophosphorus can reduce the production of thiocholine by inhibiting the activity of Acetylcholinesterase, and the corresponding raman signalNanosilver Hydrogel Surface Raman Enhanced Scattering Biosensing Technology for Detection of Paraoxon is reduced at the same time. According to the change of Raman signal, it is well achieved quantitativedetection of organophosphorus pesticides with a detectionlimitof1 ng mL-1.

Keywords： Analytical chemistry surface Raman enhanced spectroscopy hydrogel acetylcholinesterase organophosphorus pesticide