小麦生育后期叶片应答水分亏缺的转录谱特征研究
首发时间:2019-05-07
摘要:以春季不同灌水处理济麦22生育后期旗叶为材料,利用RNAseq高通量测序技术,小麦生育后期叶片应答水分亏缺的转录谱特征和差异表达基因进行了鉴定。结果表明,与正常灌水处理(W2,灌拔节和开花2水)相比,在限水灌溉处理(W1,灌拔节1水)小麦籽粒灌浆期叶片中共检测到1678个差异表达基因,其中包括1241个上调表达基因,437个下调表达基因。GO和KEGG功能注释分析表明,上述应答生育后期水分亏缺的小麦叶片差异表达基因归属于生物学过程、细胞组分和分子功能三大类别,主要参与mTOR信号通路、PI3K-Akt信号通路、乙醛酸和二羧酸代谢、氨基糖和核苷酸糖代谢、半乳糖代谢、丙酮酸代谢和淀粉及蔗糖代谢的调控。研究表明,众多功能类别基因在生育后期小麦叶片中对水分亏缺呈显著应答,参与植株对干旱胁迫的响应过程。
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Analysis on Transcriptome Characterization of Wheat Leaves during Late Growth Stage upon Water Deficit Condition
Abstract:Using the flag leaves of Jimai 22 growing at late growth stage treated by different irrigation conditions as materials, the transcriptome characterization and differentially expressed genes (DEGs) in leaves that are responses to water deficit were identified based on high-throughput RNAseq sequencing technology. Results indicated that compared with those under normal irrigation treatment (W2, two irrigations performed at jointing and flowering stages), a total of 1678 DEGs were detected in the wheat leaves during late stage under limited irrigation treatment (W1, one irrigation performed at jointing stage). Of which, 1241 DEGs were up-regulated and 437 DEGs were down-regulated. GO and KEGG analyses categorized these DEGs identified in leaves into functional groups of biological process, cell component and molecular function, which are involved in metabolic processes of mTOR signaling pathway, PI3K-Akt signaling pathway, glyoxylic acid and dicarboxylic acid metabolism, aminoglycose and nucleotide sugar metabolism, galactose metabolism, pyruvate metabolism, and starch and sucrose metabolism. These results suggested that a quantity of genes in leaf organ classified into various functional groups is modified on transcription efficiency upon water deficit condition during late growth stage, by which to mediate plant responses or adaptation to the drought stress.
Keywords: wheat limited irrigation transcriptome analysis differentially expressed genes
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小麦生育后期叶片应答水分亏缺的转录谱特征研究
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