In vitro Pollen Germination and Anther Culture of Coix lacryma-jobi L

• 1、College of Agronomy and Biotechnology, Southwest University, Chongqing 400716
• 2、 Affiliated School of Zhengzhou University, Zhengzhou 450000

Abstract：To explore job \'s-tears pollen germination in vitro condition, the establishment of job \'s-tears anther culture and callus multiplication culture system, this study adopts the 10 kinds of job \'s-tears material, by looking at different times different job \'s-tears pollen morphology, pollen vitality, pollen germination conditions were determined, period of microspore\'s shape characteristic, the results showed that the pollen grain sizes in different periods, the ellipsoid or globose, pollen vitality of 10 kinds of material in full bloom, the largest in guangdong, guangxi and hebei job \'s-tears pollen vitality;The pollen activity of sichuan white shell, punong no.1 and xingren small white shell was smaller.The optimum germination medium of pollen of coix was cultured at 25℃ for 5 ~ 7 h. The observation of the pollen grains of the early flowering stages showed that its development is continuous, with uninucleate, binucleate and trinucleate stages coexist in different ratios; the same piece of anther have microspores of different developmental stages. Microspores at the late uninucleate stage is suitable for anther tissue culture, the pollen is spherical with small vacuoles. During Coix anther culture, in the same culture medium, calluses and embryoids coexist. The anther\'s callus induction rate was in significant difference among different Coix cultivars. Calluses could be successfully induced from Xingrenxiaobaike, Zheyiyihao, Guangdong Coix, Guangxi Coix, Fujian Coix, Hebei Coix and Liaoning Boke Coix in the medium of MS+2, 4-D 1~2mg/L+KT1.5 mg/L. And Some Calluses of Coix could be successfully differentiated into short cluster buds in the medium of MS+IAA0.5mg/L+KT2 mg/L.

Keywords： Coix lacryma-jobi L Optimum Hormone Concentration Ratio Callus Test-tube Plantlets