基于金纳米模拟酶比色检测汞离子的微流控纸器件的构建和应用
首发时间:2019-11-22
摘要:本文利用金纳米模拟酶和微流控纸器件建立了一种快速方便的汞离子的比色检测方法。AuNPs可以与Hg2+结合产生类过氧化物酶活性,能够催化能够催化过氧化氢(H2O2)和四甲基联苯胺(TMB)的显色反应,由无色变成蓝色。将AuNPs,Hg2+溶液和显色底物(TMB和H2O2的混合溶液)分别滴加在纸器件的样品检测区和显色分配区。同时通过叠加滤纸的方法,实现Hg2+浓度的富集,进而提高检测的灵敏度。反应后可直接肉眼识别颜色变化,扫描记录实验结果。用Image J进行灰度分析,建立了定量检测Hg2+的方法,其线性范围是50-2000 nmol/L,检出限为17 nmol/L,肉眼可以识别出的最低浓度为50 nmol/L。此外,该检测方法也应用于自来水和湖水样品的检测,回收率在80%-120%之间证明方法的可行性。
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Microfluidic Paper-based Device for Colorimetric Detection of Mercury Ions Using Gold Nanozyme
Abstract:In this paper we developed a facile and convenient microfluidic paper-based device for colorimetric detection of mercury(Hg2+) by using gold nanozyme. The colorimetric detection of Hg2+ is established based on the peroxidase-like catalytic activity of gold nanoparticles promoted by Hg2+ , which is correlated to the colorimetric intensity from the catalytic reaction of TMB and H2O2. AuNPs and Hg2+ solution were dropped on detection zones of the paper-based device. Chromogenic enzyme substrates (mixed solution of TMB and H2O2) were dropped on distribution zones of the paper-based device. By stacking the filter paper, the volume of the Hg2+ solution increased to act to filter and concentrate regents and improve the sensitivity of the detection. The assay results were recorded by scanner and then colorimetric signal analysis was carried out using ImageJ software. The linear range of Hg2+ detection was 50-2000 nmol/L and the detection limit was 17.0 nmol/L. The minimum concentration of Hg2+ that can be recognized by the naked eye was 50 nmol/L. In addition, the paper-based device was also applied to the detection of tap water and lake water samples, and the recovery was between 80% and 120%. These results indicate that the paper-based device is reliable and suitable for practical applications.
Keywords: paper-based device mercury ion gold nanoparticles colorimetric detection
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基于金纳米模拟酶比色检测汞离子的微流控纸器件的构建和应用
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