CH-X Preconditioning Significantly Decreased the High Mortality Rate in a Rat Model of Acute Post-Infarction Heart Failure(Breakthrough)

• 1、Circulatory and Body Fluid Regulation, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan

Abstract：Background: The rat model of post-infarction heart failure is of great value in experimental study of cardiology. However, the biggest disadvantage of this disease model is the extremely high acute mortality rate. This article briefly introduces the experimental results of a new scenario where a significant decrease was seen in the mortality rate in a rat model of acute post-infarction heart failure. Nevertheless, the scientificalness of the existing popular pharmacodynamic papers using the same modeling approaches is being furtherly discussed following the study of more than 800 AMI rat models. Method: CH-X preconditioning was an accidental finding in the building of acute myocardial infarction (AMI) model in rats. Ligation was performed on the very proximal position of the left coronary artery in male Wistar rats weighing 220-320g, to ensure a large area of myocardial infarction. Two groups of experimental data were collected in chronological order: The first was 2005-12-AMI-21-rats group and was observed for the next 70 days. The second was from a repeatable study of 2008-05-AMI-23-rats group and was observed for the following 285 days. The extent of myocardial infarction was assessed after spontaneous death or eventual death. Then it was compared to the results of a classic paper from harvard in 1995. Results: The proportions of large area myocardial infarction (about 50%) in the two groups were 17/21 and 19/23 respectively, the mortality rate in 24 hours of the two groups was 23.8% (5/21) in the 2005-12-AMI-21-rats group and 17.4% (4/23) in the 2008-05-AMI-23-rats group, the half lethal time was 40 days for the former group and 60 days for the latter group. In sharp contrast, the mortality rate of the 1995-harvard-26-AMI-rats group was 69.2% (18/26) in 24 hours, which indicated that the mortality rate of acute myocardial infarction was reduced significantly by about 3 times, and the half lethal time was only 9 hours (<1day) then. Conclusion: CH-X preconditioning is a very simple and effective measure, which can significantly reduce the mortality rate of the disease model of heart failure induced by myocardial infarction in rats. The improved survival rate for this important heart failure disease model would increase cost efficiency and ethical acceptance significantly. In addition, Both the methodology involved in the disease model and its modeling feasibility are in need of further level of rigorous assessment.

Keywords： CH-X(China-X) MI(Myocardial Infarction) HF(Heart Failure) Mortality Half lethal time