去肌腱对骨骼肌碳酸酐酶3的影响

黄河; 任惠民; 刘学源

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去肌腱对骨骼肌碳酸酐酶3的影响

首发时间：2019-11-28

黄河 ¹
黄河（1982.6-），男，主治医师，脑血管病和神经肌肉疾病
任惠民 ² 刘学源 ³
刘学源（1962.10-），男，主任医师、博导，神经退行性疾病

1、同济大学附属上海市第十人民医院神经内科
2、复旦大学神经病学研究所
3、同济大学附属上海第十人民医院神经内科

摘要：目的：探讨去肌腱对碳酸酐酶III CAIII表达和磷酸酶活性的影响。方法：对大鼠趾长伸肌EDL和比目鱼肌Sol去肌腱，取术后7d、14d、28d和56d的肌肉，采用相邻切片分别进行ATPase染色和CAIII免疫组化染色，Western Blot分析CAIII蛋白，固相膜上原位酶活性分析CAIII磷酸酶活性。结果：去肌腱的Sol组织形态无显著变化，而EDL中的快纤维逐渐肥大。相邻切片染色显示，去肌腱Sol和EDL的肌纤维ATPase染色和CAIII免疫染色对应关系不变，即CAIII免疫染色着色纤维即ATPase染色的I型纤维。Western Blot分析表明，去肌腱侧Sol和EDL的CAIII蛋白表达水平高于对照侧，但前者CAIII磷酸酶活性低于后者，且去肌腱Sol的CAIII磷酸酶活性下降更显著。结论：骨骼肌CAIII表达水平及磷酸酶活性与骨骼肌主动运动和运动模式有关。

关键词：骨骼肌去肌腱碳酸酐酶III 碳酸酐酶III磷酸酶活性

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Effects of tenotomy on carbonic anhydrase 3

HUANG He ¹
黄河（1982.6-），男，主治医师，脑血管病和神经肌肉疾病
REN Huimin ² LIU Xueyuan ¹
刘学源（1962.10-），男，主任医师、博导，神经退行性疾病

1、Department of Neurology, Tenth People’s Hospital, Tongji University School of Medicine, Shanghai200072, P.R. China
2、Institute of Neurology, Fudan University, Shanghai 200040, P.R.China

Abstract：AIM/OBJECTIVE: To investigate the effects of tenotomy on the expression and phosphatase activity of carbonic anhydrase III (CAIII). METHODS: Tendons of extensor digitorum longus (EDL) and soleus muscle(Sol) were removed from the rats. The operated and contralateral muscles were taken at 7d, 14d, 28d and 56d after operation. ATPase staining and CAIII immunohistochemical staining were performed on adjacent sections. Western Blot was used to analyze the expression of CAIII, and phosphate activity analysis on the nitrocellulose filter following electrophoresisin was applied for CAIII phosphatase. RESULTS: There was no significant change in the morphology of the Sol after tenotomy, but the fast fibers in the operated EDL became hypertrophied gradually. Adjacent slice staining showed that the relationship between ATPase staining and CAIII immunostaining of Sol and EDL after tenotomy was unchanged, that is, CAIII immunostaining colored fiber was identified as type I fiber by ATPase staining. Western Blot analysis showed that the expression of CAIII in operated Sol and EDL was higher than that in the control side; contrarily, the CAIII phosphatase activity of the former was lower than that of the latter, especially for the operated Sol. CONCLUSION: The expression and phosphatase activity of CAIII in skeletal muscle are related to the movement and affected by activity patterns.

Keywords： Skeletal muscle tenotomy carbonic anhydrase III phosphate activity

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黄河，任惠民，刘学源. 去肌腱对骨骼肌碳酸酐酶3的影响[EB/OL]. 北京：中国科技论文在线 [2019-11-28]. https://www.paper.edu.cn/releasepaper/content/201911-90.

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论文编号	201911-90
论文题目	去肌腱对骨骼肌碳酸酐酶3的影响
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