一种便捷高效经济的细胞运输策略
首发时间:2020-02-07
摘要:目的:开发一种简单高效、安全无毒且经济环保的细胞运输方法,以便研究人员在全球范围内实现常温条件下的细胞运输。方法:将细胞重悬于100%的胎牛血清(Fetal bovine serum,FBS)中在常温条件下进行运输。对小鼠胚胎干细胞(mESCs)、Hela、MEF、A549、293T、MS1、J774A.1和Huh7八种实验室常用细胞系进行该新方法和传统干冰运输方法的比较。具体如下:我们将待运输的细胞消化为单细胞悬液,离心收集沉淀,重悬于装有800-1000微升FBS的Eppendorf管中,分别在4℃、20℃及30℃条件下储存细胞3天。另一份作为对照采用传统方法,储存于含10%DMSO的培养基中在干冰中保存三天。通过MTT检测细胞的存活率,并通过对复苏后的细胞计数并绘制生长曲线,检测细胞的增殖能力,以比较不同细胞的运输条件的效果。结果:与传统方法相比,采用新方法运输的细胞在细胞形态、增殖能力、细胞活性及细胞特性等方面未见明显差异,部分类型细胞常温运输复苏后细胞活性提高。结论:该方法可在常温下进行,无需干冰的消耗,简单安全、高效且经济环保。采用此方法可更便捷高效地在各地区间进行细胞运输。
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A Convenient, Efficient and Economical Cell Transportation Strategy
Abstract:Objective: To develop a simple, efficient, safe and environment-friendly cell transportation method with low cost, so that researchers can deliver cell samples under ambient temperatures in the globe. Methods: Cells were resuspended in 100% Fetal bovine serum (FBS) and transported at ambient temperatures. The new method is compared with the traditional transportation for shipping eight cell lines commonly used in laboratories, which are mouse embryonic stem cells (mESCs), Hela, MEF, A549, 293T, MS1, J774A.1 and Huh7. Specifically, we re-suspended cells in 800-1,000 microliters of FBS in Eppendorf tubes, and kept at 4℃, 20℃ and 30℃ for 3 days respectively. For the conditional method, cells were kept in medium with 10% DMSO in dry ice for 3 days. The survival rate of cells was measured by MTT, and the proliferation ability of cells was assayed by counting the cell number and plotting the growth curve to compare the effects of different cell transportation conditions. Results: Compared with the traditional method, the cells transported by the new method showed no obvious difference in cell morphology, proliferation ability, cell viability and other cell characteristics, and the cell viability of some types of cells increased after ambient temperature transportation. Conclusion: Cell sample transportation can be carried out globly at ambient temperatures without consumption of dry ice. By adopting the method, cell samples could be shared more economically and efficiently.
Keywords: Cell transportation Dry ice FBS
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