锰过氧化物酶的酵母重组表达及其降解黄曲霉毒素B1研究
首发时间:2020-02-13
摘要:本研究构建了食品级重组乳酸克鲁维酵母菌株GG799(pKLAC1-Phsmnp)、GG799(pKLAC1-Plomnp)和GG799(pKLAC1-Phcmnp)。实现了三种不同来源锰过氧化物酶在乳酸克鲁维酵母中的异源表达,并且比较了三种重组乳酸克鲁维酵母菌发酵上清液对于黄曲霉毒素B1的降解效果,发现GG799(pKLAC1-Phcmnp)对于黄曲霉毒素B1的降解效果最好,达到50.52%。本研究还优化了重组菌株GG799(pKLAC1-Phcmnp)的诱导表达条件,在经正交试验优化后得到的最佳诱导条件下,重组菌发酵上清液对AFB1的降解率能达到67.4%,与优化前相比,降解率提高了16.88%,与单因素优化试验最优结果相比,降解率提高了9.08%。
关键词: 黄曲霉毒素B1 锰过氧化物酶 乳酸克鲁维酵母 异源表达
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Study on Yeast Regeneration Expression of Manganese Peroxidase and Its Degradation of Aflatoxin B1
Abstract:In this study, food-grade recombinant Kluyveromyces lactis strains GG799 (pKLAC1-Phsmnp), GG799 (pKLAC1-Plomnp), and GG799 (pKLAC1-Phcmnp) were constructed.Heterologous expression of three different sources of manganese peroxidase in Kluyveromyces lactis was achieved, and the degradation effects of three recombinant Kluyveromyces lactis fermentation supernatants on aflatoxin B1 were compared.It was found that GG799 (pKLAC1-Phcmnp) had the best degradation effect on aflatoxin B1, reaching 50.52%.This study also optimized the inducible expression conditions of the recombinant strain GG799 (pKLAC1-Phcmnp). Under the optimal induction conditions optimized by orthogonal experiments, the degradation rate of AFB1 by the recombinant fermentation supernatant could reach 67.4%. Compared with the previous one, the degradation rate was increased by 16.88%, and compared with the single factor optimization, the degradation rate was increased by 9.08%.
Keywords: Aflatoxin B1 Manganese peroxidase Kluyveromyces lactis Heterologous expression
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锰过氧化物酶的酵母重组表达及其降解黄曲霉毒素B1研究
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