The effects of coactosin-like protein knockout on allergic asthma

• 1、Food School, Jiangnan University, Wuxi 214100
• 2、Medicine School, Jiangnan University, Wuxi 214100

Abstract：Coactosin-like protein (CLP) is an F-actin-binding protein that can also function as a scaffold for 5-lipoxygenase (5LO). A role for CLP in regulating 5LO and leukotriene (LT) formation has been described previously. However, how CLP might influence the immune response in allergic asthma is unknown. We approached this question by generating a CLP deficient mouse model. The results showed that clp-deficient mice had more immune cell infiltration in bronchoalveolar lavage (BAL) and higher levels of Th2 inflammatory cytokines after asthma than wild-type asthma mice. Histomorphological analysis showed that the bronchial contraction was more severe and mucin was more secreted in the respiratory epithelium of clp-deficient asthma mice. The results of lipid analysis in BAL proved the regulation ability of CLP to eicosanoids. Analysis of proteases and receptors associated with eicosanoids also showed that CLP deficiency led to up-regulation of prostaglanin D2 (PGD2) synthase (HPGDS) and receptor (CRTH2) in asthmatic mice. In summary, this study shows that lack of CLP leads to worsening of asthma in mice, and this deterioration is associated with PGD2.

Keywords： allergic asthma, coactosin-like protein, eicosanoids, prostaglandin