表面增强拉曼散射用于金黄色葡萄球菌生物被膜的原位检测
首发时间:2020-03-13
摘要:生物被膜在自然界以及医学和工程领域普遍存在,这种细菌聚集形成的群落难以被彻底清除与杀灭,会造成反复污染,因此接触表面被膜的原位检测技术十分重要。本研究拟开发应用操作简便、快速、灵敏度高的表面增强拉曼散射(SERS)检测新技术对生物被膜进行原位检测,PVC载片在模拟管道中培养不同天数后,对生长在载片上的生物被膜分别用传统紫外法、共聚焦激光扫描显微镜(CLSM)法和SERS法进行了检测。实验结果表明,随着培养天数的增加生物被膜不断增加,I1172 cm-1/I722 cm-1和I1384 cm-1 /I722cm-1的相对峰强与时间都成正相关,线性拟合后R2分别为0.9576,0.9640,说明这两处结晶紫的特征峰能够较好的反映生物被膜的变化趋势;与传统的紫外法和CLSM法进行皮尔逊相关分析,结果表明SERS法与紫外法具有较好的相关性(p<0.05),与CLSM法具有显著相关性(p<0.01),说明了SERS法作为检测生物被膜膜量变化是一种可靠的新技术。
关键词: 金黄色葡萄球菌 生物被膜 表面增强拉曼光谱 结晶紫
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Surface enhanced Raman scattering for in situ detection of Staphylococcus aureus biofilms
Abstract:Biofilms are ubiquitous in nature and in the fields of medicine and engineering. It is difficult to completely remove and kill the communities formed by the accumulation of bacteria, which will cause repeated pollution. Therefore, the in situ detection technology of the contact surface biofilm is very important. This study intends to develop a simple, fast, and highly sensitive surface enhanced Raman spectroscopy (SERS) detection technology for in situ detection of biofilms. After the PVC slides were cultured in the simulated pipeline for different days, the biofilm grown on the slides were detected by traditional ultraviolet method, confocal laser scanning microscope (CLSM) method and SERS method respectively. The experimental results showed that with the increase of the number of culture days, the biofilm increased, and the relative peak intensities of I1172 cm-1 / I722 cm-1 and I1384 cm-1 / I722cm-1 were positively correlated with time, after linear fitting, R2 were 0.9576 and 0.9640, respectively, indicating that the characteristic peaks of the crystal violets can better reflect the change trend of the biofilm; Pearson correlation analysis with the traditional UV method and CLSM method has shown that the SERS method has a good correlation with the UV method (P <0.05), which has a significant correlation with the CLSM method (p <0.01), which shows that the SERS method is a reliable new technique for detecting changes in the amount of biofilm.
Keywords: Staphylococcus aureus biofilm SERS crystal violete
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表面增强拉曼散射用于金黄色葡萄球菌生物被膜的原位检测
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