Cur@Hb nanoparticles enhance radiosensitivity of hypoxic hepatocellular carcinoma cells

• 1、School of Radiological Medicine and Protection, Medical College of Soochow University

Abstract：Malignant tumors have become an important cause of harm to national health, and radiation therapy has long been an important part of tumor treatment. However, hypoxia is common in malignant solid tumors, and the resistance of hypoxic tumor cells to radiation therapy is increased, which seriously affects the efficacy of tumor radiation therapy. Incubation Cur@Hb in normoxia or hypoxia (1% O2) for 24 h can significantly inhibit the proliferation and migration of SMMC 7721 cells, and promote apoptosis. Cur@Hb combined with 4 Gy X-ray irradiation caused G2/M phase arrest of SMMC7721 cells. In addition, Cur@Hb nanoparticles can significantly inhibit the generation of vascular tube-like structures, and the expression of EMT-related protein VE-cadherin, twist1 and MMP2 was significantly decreases.Cur@Hb nanoparticles significantly enhanced the radiosensitivity of normoxic and hypoxic SMMC7721, with radiosensitization ratios of approximately 1.385 and 1.309, respectively; Cur@Hb nanoparticles significantly increased the x-ray-induced percent of DNA tail of normoxic or hypoxic SMMC7721 cells quantity. Cur@Hb nanoparticles can significantly enhance the effect of radiotherapy.

Keywords： hepatocellular carcinoma hypoxia radiosensitivity