Preparation, characterization and osteogenesis of calcined bovine bone modified with HA and CS

• 1、School/Hospital of Stomatology, Lanzhou University, Lanzhou 730000

Abstract：Objective: To study the characterization and osteogenic ability of the surface of calcined bovine bone modified by hydroxyapatite and chitosan, in order to prepare a safe and nontoxic scaffold material with good physical and chemical properties. Methods: A layer of HA mineralized substance was formed on the surface of calcined bovine bone immersed in simulated body fluid (SBF), and then soaked in chitosan solution to make CS fully attached to the surface.The energy spectrum analyzer analyzes the Ca/P ratio of the minerals on the surface of the material, and porosity and mechanical properties of the material was detected. The porosity and mechanical properties of the materials were tested; Based on BMSCs, the biological safety and cytocompatibility of BMSCs were tested by cytotoxic and adhesion tests; The expression of ALP was detected by ELISA kit, and the mRNA expression of col Ⅰ, OPN and OCN was detected by qRT-PCR. Results: The main component of calcined bone is β-TCP, and the main component of surface mineralized substance is HA. The main component of the calcined bone modified by HA/CS was β-TCP/HA/CS. There was no significant difference in porosity between them. The mechanical properties of β-TCP/HA/CS materials were significantly improved (P<0.01). The proliferation and differentiation of BMSCs were promoted, the expression of ALP protein was significantly increased (P<0.01), and the mRNA expression levels of COL Ⅰ, OPN and OCN genes were significantly increased (P<0.01). Conclusion: β-TCP/ HA/CS is a safe, nontoxic and mechanical composite scaffold material with good osteogenesis induction.

Keywords： Calcined bone Bionic mineralization HA CS BMSCs