Coupled Enrichment and MALDI-TOF-MS Analysis of Vicinal Diols Using As-Prepared Boron Nanosheets as Matrix

• 1、School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331

Abstract：MALDI-TOF-MS is commonly used to detect large molecular compounds such as proteins, peptides, polymers, etc. This method has the advantages of high sensitivity, fast detection speed, convenient sample preparation, high salt tolerance, and low sample requirements. In order to increase the detection range of this method, a new type of nano-matrix came into being. Examples include graphene, carbon dots, porous silicon, and metal nanoparticles. Although these nanomaterials have good matrix properties and can avoid the interference caused by the traditional matrix itself, they have the disadvantages of complex preparation, difficult batch synthesis, and cannot be used for enrichment and detection at the same time. This paper uses a top-down synthesis method to synthesize boric acid-functionalized two-dimensional boron nanosheets (2DBS). This method is convenient, requires low equipment, simple preparation, easy batch preparation, and high product purity. The experiments show that the synthesized 2DBS has obvious ultraviolet absorption near the 350 nm wavelength, which provides conditions for 2DBS as a matrix. In the positive ion mode, the small molecule compounds such as carbohydrates and amino acids are used for MALDI-TOF-MS analysis, which has a high detection sensitivity. Due to the presence of borate functional groups, it is also possible to specifically enrich carbohydrates with vicinal diols structure, which has excellent enrichment effects and can simultaneously achieve the purpose of enrichment and detection.

Keywords： 2DBS MALDI-TOF-MS carbohydrates enrichment and detection