双水相从黄芩中同时提取和分离黄芩苷和糖

袁小琳; 穆小静; 李颜; 苟艳; 唐美伶; 肖尚友

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Simultaneous extraction of baicalin and saccharides from Scutellariae Radix with aqueous two-phase system

首发时间：2020-05-06

Yuan Xiaolin ¹
Yuan Xiaolin(1995), female, separation and purification of natural products
Mu Xiaojing
Mu Xiaojing(1973), female, associate professor, pharmacy & biochemistry
Li Yan Gou Yan Tang Meiling Xiao Shangyou

1、Department of Pharmaceutical Engineering, College of Chemistry & Chemical Engineering, Chongqing University, Chongqing, China, 401331

Abstract：Scutellariae Radix is a Chinese herb for detoxification. Efficient extraction of its main active components baicalin and saccharides is interesting to pharmaceutical and cosmetic fields. The baicalin and saccharides were efficiently extracted with aqueous two-phase systems (ATPSs) Ethanol-(NH4)2SO4-H2O (EtSO) and Ethanol-cosolvent-(NH4)2SO4-H2O (EtSO-cosolvent). The factors such as pH, (NH4)2SO4 concentration, ethanol fraction, cosolvent fraction, extraction time and temperature were optimized and thermodynamics for partition was discussed. With the extractions at 48 C for 40 min, the EtSO, EtSO-MtOH and EtSO-Acetone exhibited higher baicalin yield than conventional 70% ethanol system (v/v) by 21.4%, 24.8% and 26.2%, respectively; and they displayed saccharides yield in a similar level to that with water system. By dialysis method the saccharides extracted with ATPS was proved to have similar molecular weight to those extracted with water. Therefore, it is feasible to obtain two birds (two targets) with one stone (ATPS) in high efficiency.

keywords： Aqueous two-phase system two-component extraction Scutellariae Radix baicalin saccharides

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双水相从黄芩中同时提取和分离黄芩苷和糖

袁小琳 ¹
Yuan Xiaolin(1995), female, separation and purification of natural products
穆小静
Mu Xiaojing(1973), female, associate professor, pharmacy & biochemistry
李颜苟艳 唐美伶 肖尚友

1、重庆大学化学化工学院，重庆市，401331

摘要：黄芩是一种清热解毒的中草药。其主要活性成分黄芩苷和糖类的高效提取是医药和化妆品领域的研究热点。用双水相系统（ATPSs）乙醇-硫酸铵-水（EtSO）和乙醇-助溶剂-硫酸钠-水（EtSO-助溶剂）有效提取黄芩苷和糖类。优化了pH，硫酸铵浓度，乙醇体积分数，助溶剂组成，萃取时间和温度等因素，并讨论了分配的热力学。在48℃下萃取40分钟后，EtSO，乙醇-甲醇-硫酸铵-水（EtSO-MtOH）和乙醇-丙酮-硫酸铵-水（EtSO-Ac）的黄芩苷收率分别比常规70％乙醇体系（v / v）高21.4％，24.8％和26.2％。并且它们提取的糖产量与水系统提取产量相似。通过透析，证明用双水相提取的糖具有与用水提取的糖相似的量。因此，用一步提取（ATPS）高效地获得两个目标组分是可行的。

关键词：双水相两组分萃取黄芩黄芩苷糖类

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Yuan Xiaolin,Mu Xiaojing,Li Yan,et al. Simultaneous extraction of baicalin and saccharides from Scutellariae Radix with aqueous two-phase system[EB/OL]. Beijing:Sciencepaper Online[2020-05-06]. https://www.paper.edu.cn/releasepaper/content/202005-1.

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论文编号	202005-1
论文题目	双水相从黄芩中同时提取和分离黄芩苷和糖
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