基于正交反应激活的DNAzyme活性调控
首发时间:2020-05-19
摘要:我们开发了一种基于正交反应激活的DNAzyme的活性调控的新方法。该DNAzyme为RNA切割式DNAzyme,底物链上有且只有一个RNA,在特定金属离子辅助下具有特异性切割RNA底物的活性。DNAzyme底物链在和苯硼酸酯酰化试剂反应后,该底物链上唯一的RNA被修饰,导致组装后的DNAzyme处于失活状态。苯硼酸酯基团对H2O2具有高灵敏度和高选择性,因此只有在H2O2存在条件下恢复活性。在细胞成像过程中,稳定性提高,背景大大降低,且由于癌细胞中H2O2浓度较正常细胞高很多,可实现在空间上对DNAzyme进行活性调控。本章所开发的策略可以为DNAzyme内源性活性调控提供宝贵的平台。
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Research on the regulation of DNAzyme activity based on Orthogonal Activation
Abstract:we developed a new method for the active regulation of DNAzymes based on orthogonal activation. The DNAzyme is an RNA-cleaving DNAzyme, and there is only one RNA on the substrate strand, which has the activity of specifically cleaving RNA substrates with high specificity for a specific metal ion of interest. After the DNAzyme substrate chain reacts with the phenyl borate acylation reagent, the only RNA on the substrate chain is modified, resulting in the inactivated state of the assembled DNAzyme. The phenyl borate group has high sensitivity and high selectivity to H2O2, so it can only restore activity in the presence of H2O2. In the process of cell imaging, the stability is improved, the background is greatly reduced, and because the concentration of H2O2 in cancer cells is much higher than that of normal cells, it is possible to spatially regulate the activity of DNAzymes. The strategy developed in this chapter might provide a valuable platform for the regulation of DNAzyme endogenous activity.
Keywords: DNAzyme;Phenyl borate group H2O2;orthogonal activation
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