Design, Synthesis and Properties of FAP Probe

• 1、College of chemistry and chemical engineering, Hunan University, Changsha 410000, China

Abstract：In this paper………..Biological protease plays an important role in human body, and participates in metabolism and synthesis of important substances. At present, it has been proved that enzymes are closely related to the production of many diseases and play an important role in the abnormal metabolism and expression of human physiology. FAP (Fibroblast Activation Protein) is a serine protease, which exists in the matrix of fibroblasts and expresses abnormally. The high expression in many tumor cells， meanwhile development of these tumor cells. The detectiion of FAP has become a necessary research work. Therefore, this paper can design, synthesize and detect FAP, and the mechanism of cleavage of amide bond at the end of protein by FAP, we chose Cbz-Gly-Pro-OH as the response peptide, the fluorescein dye as the fluorescent group. At one end of the dye, the protein is linked by p-aminobenzyl alcohol, and the other end is condensed to make the dye red shift, near infrared dye fluorescence probe (NIR-FAP) for FAP detection in vitro and cell imaging was designed and synthesized, and the detectiong limit of probe is 1.0 U/L.

Keywords： analytical chemistry fluorescein NIR-FAP