The inhibition of BifidobacteriumLongum FSCDJY6M1 against Penicilliumexpansum and the analysis of its antifungal metabolites

• 1、School of Food Science and Technology, Jiangnan University,Wuxi, Jiangsu, 214122

Abstract：The contamination of Penicillium expansum of fruits causes tissue spoilage which leads to great commercial losses and the mycotoxin residues in fruits and fruit-by products further threaten human health. Currently, lacic acid bacteria has been widely used to food fermentation and the elongation of shelf period, however, its antifungal ability against P. expansum is still rarely explored. [Aim] Evaluate the inhibition of Lactobacillus and Bifidobacterium against P. expansum, screen out the specific bacterial strain with highly inhibitory ability against P. expansum, study its antifungal activity, and analyze the potential antifungal compounds. [Methods] Dual-culture overlay assay, microtiter plate well assay, and agar spot assay were successively performed to screen and verify the antifungal activity of Lactobacillus and Bifidobacterium separated from fermented food or human feces; pH, heat, and enzyme treatments were conducted to study the antifungal activity of cell-free supernatant; HPLC, as well as GC-MS, were used to identify the potential antifungal compounds. [Results] The antifungal ability of Lactobacillus and Bifidobacterium against P. expansum are species- and strain-specific. Bifidobacterium longum FSCDJY6M1 can effectively inhibit the growth of P. expansum and the production of patulin with mainly organic acids and some non-protein components. [Conclusion] B. longum FSCDJY6M1 effectively inhibit the pollution of P. expansum, indicating its potential as a novel biocontrol treatment to prevent or delay common fungal spoilage.

Keywords： Lactobacillus Bifidobacterium Penicillium expansum biological control