The relationship between yeast Whi2 and Msn2/Msn4 during the regulation of autophagy induction

• 1、College of pharmaceuticle Science,Soochow University, Suzhou 215000

Abstract：Aim: In this study, we investigated the relationship between Whi2 and Msn2/Msn4 during the regulation of yeast autophagy induction. Methods: The prATG8-GFP plasmid was used to detect the transcription level of the autophagy gene ATG8 under specific nutritional conditions, and the expression level of GFP indicated by western blot indirectly reflected the level of intracellular autophagy induction. Yeast strains with deleted genes were constructed by homologous recombination. Yeast strains overexpressed with certain proteins were obtained through transformation with specific plasmids. The results were analyzed according to the epistasis analysis to map the signaling pathway. Results: Knocking out MSN2 or MSN4 alone did not change the transcription level of ATG8 The relationship between yeast Whi2 and Msn2/Msn4 during the regulation of autophagy inductionin the cells, while knockinThe relationship between yeast Whi2 and Msn2/Msn4 during the regulation of autophagy inductiong out both MSN2 and MSN4 caused significant decrease of the ATG8 transcription level. The ATG8 transcription levels in Δmsn2Δmsn4 and Δwhi2 strains are similar. Overexpression of Msn2 or Msn4 in Δwhi2 cells can increase the transcription level of ATG8, both under low leucine condition and nitrogen deprivation condition. Conclusion: Msn2 has functional similarity with Msn4 in promoting autophagy induction. Msn2/Msn4 is downstream of Whi2, and does not depend on Whi2 to promote autophagy induction.

Keywords： Microbial and Biochemichal Pharmacy yeast Whi2 Msn2/Msn4 transcription factors autophagy ATG8 transcription