微胶囊化肉桂醛的制备与抑菌稳定性研究
首发时间:2020-05-29
摘要:随着人们生活水平和安全意识的不断提高,寻求安全、高效、绿色的天然抑菌剂已成为趋势。肉桂醛是肉桂精油的活性成分物质,作为抑菌剂可以满足食品安全的要求。但是由于肉桂醛易挥发、不稳定,导致其长效抑菌效果差,限制了其应用。通过微胶囊技术可以保护肉桂醛、延长其抑菌效果。本文将巴西曲霉作为试验菌种,采用复合凝聚法制备肉桂醛微胶囊,研究壁材组成、芯壁比、交联剂对微胶囊化效率和抑菌速效性的影响。确定肉桂醛微胶囊的最佳制备工艺为:以明胶/阿拉伯胶为壁材,芯壁比2:1,单宁酸作为交联剂,单宁酸添加量为0.3%。肉桂醛的微胶囊化效率达92.53±5.20%,抑菌圈为37.82 ± 0.63 mm,微胶囊化效率高,抑菌效果好。在加速贮存期间,微胶囊可以有效保护肉桂醛,延缓保留率的降低,减少贮存期间抑菌性损失,提高肉桂醛抑菌稳定性。通过测定抑菌机理结果可知,未微胶囊化肉桂醛和微胶囊化肉桂醛都通过破坏菌丝细胞膜,导致胞内蛋白泄露的方式抑制霉菌生长。
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Study on preparation and antifungal stability of microencapsulated cinnamaldehyde
Abstract:With the continuous improvement of people\'s living standards and safety awareness, it has become a trend for people to seek safe, efficient and green natural preservatives. Cinnamaldehyde is the active ingredient of cinnamon essential oil. As an antifungal agent, it can meet the requirements of food safety. However, cinnamaldehyde is volatile and unstable, resulting in poor long-term antifungal effect, which limits its application. Microcapsule technology can protect cinnamaldehyde and prolong its antifungal effect. In this paper, Aspergillus brasiliensis was used as a test strain, and cinnamaldehyde microcapsules were prepared by a composite coacervation method to study the effects of wall material composition, core-to-wall ratio, and crosslinking agent on microencapsulation efficiency and antifungal activity. The best preparation process of cinnamaldehyde microcapsules was determined by using Gelatin / Arabic gum as the wall material, core-to-wall ratio of 2: 1, tannic acid as the cross-linking agent, and the amount of tannic acid added was 0.3%. The encapsulation efficiency of cinnamaldehyde was 92.53 ± 5.20%, and the inhibitory zone diameter was 37.82 ± 0.63 mm. The microencapsulation efficiency of cinnamaldehyde is high and the antifungal effect is good. During accelerated storage, microcapsules can effectively protect cinnamaldehyde, delay the decrease of retention rate and improve the cinnamaldehyde bacteriostatic stability. By measuring the antifungal mechanism, it can be known that both non-microencapsulated and microencapsulated cinnamaldehyde inhibit the growth of fungi by destroying the mycelial cell membrane and causing intracellular protein leakage.
Keywords: Microcapsule Cinnamaldehyde Antifungal activity Microencapsulation efficiency
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