Hand-powered centrifugal microfluidic chip fot detecting marathion

• 1、College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082

Abstract：Detecting organophosphorus pesticide by a portable device that do not require electricity is very important for food safety in areas with limited resources. Inspired by the whirligig, we developed a hand-powered centrifugal microfluidic chip for rapid and portable detection of the organophosphorus pesticide, malathion. We encapsulated acetylcholinesterase (AChE) and chromogenic agent indole acetate (IDA) within pullulan to form the films and pre-stored them in the enzyme inhibition zone and the color development zone of the microfluidic chip, respectively. After adding the sample to the enzyme inhibition zone for a period of time, we inserted chip into the disc. Under the centrifugal force generated by pulling the disc, the solution in the enzyme inhibition zone was transferred to the color development zone. Finally, we measured the content of organic phosphorus pesticides in the sample by observing the color intensity of the color development zone. The linear detection range of the device to detect marathion is 0.1-20 μmol/L, and the detection limit is 0.1 μmol/L. The hand-powered device without external power supply has important application potential in field detection.

Keywords： Analytical chemistry hand-powered microfluidic chip malathion