Au@Ag核壳纳米材料的抗菌性能和细胞毒性研究
首发时间:2020-06-15
摘要:细菌感染严重威胁人类健康,抗生素的广泛过度使用引发"超级细菌"的产生。因此,迫切需要安全有效的抗菌剂来替代常规抗生素。银纳米颗粒(Ag NPs)已被证明具有广谱抗菌性,但是它的细胞毒性限制了其在生物医学领域应用。Au@Ag核壳纳米粒子由于双金属的协同作用,杀菌能力相比于Ag NPs显著提高。已有研究表明Ag NPs的细胞毒性具有尺寸效应,即粒径越小毒性越大。但是,关于Au@Ag核壳纳米粒子的粒径大小对细胞毒性和杀菌性能影响的研究较少。我们制备了三种粒径大小、颗粒均匀的Au@Ag核壳纳米粒子,并研究了三者对大肠杆菌和金黄色葡萄球菌的抗菌性能以及对L929小鼠成纤维细胞的毒性。结果表明,小粒径的Au20@Ag核壳纳米粒子的杀菌能力最强,Au50@Ag次之,Au80@Ag最小。但是Au20@Ag核壳纳米粒子细胞毒性远大于Au50@Ag和Au80@Ag,后两者毒性差异很小。这些发现对设计应用于生物医药领域的Au@Ag核壳纳米粒子具有重要指导意义。
关键词: Au@Ag核壳纳米粒子 抑菌性能 细胞毒性 粒径
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Study on antibacterial properties and cytotoxicity of Au@Ag core-shell nanoparticles
Abstract:Bacterial infections seriously threaten human health, and widespread overuse of antibiotics has triggered the emergence of "superbug". Therefore, there is an urgent need for safe and effective antibacterial agents to replace conventional antibiotics. Researches have shown that silver nanoparticles (Ag NPs) have broad-spectrum antibacterial properties, but their cytotoxicity limits their application in the biomedical field. Due to the synergistic effect of bimetals, the sterilization ability of Au@Ag core-shell nanoparticles is significantly improved compared to pure Ag NPs. Studies have shown that the cytotoxicity of Ag NPs has a size effect, the smaller the particle size, the greater the toxicity. However, the effect of the particle size of Au@Ag core-shell nanoparticles on cytotoxicity and bactericidal properties is less studied. We prepared three Au@Ag core-shell nanoparticles with uniform particle size, and studied their antibacterial properties against E.coli and S.aureus and their cytotoxicity against L929 mouse fibroblasts. The results show that the small-diameter Au20@Ag core-shell nanoparticles have the strongest antibacterial ability, followed by Au50@Ag, and Au80@Ag the worst. But the cytotoxicity of Au20@Ag core-shell nanoparticles is much greater than that of Au50@Ag and Au80@Ag, and the latter two have very little difference in cytotoxicity. These findings have important guiding significance for the design of Au@Ag core-shell nanoparticles applied in the field of biomedicine.
Keywords: Au@Ag core-shell nanoparticles Antibacterial properties cytotoxicity Particle size
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