Multiple detection methodologies of new coronavirus SARS-CoV-2

• 1、School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022

Abstract：Since December 2019, the global new crown epidemic has erupted. As of June 25, 2020, 9,350,845 people have been diagnosed overseas and 476,532 people have died. A total of 85,104 people have been diagnosed in our country and 4,647 people have died. WHO has warned that the new global epidemic situation is still worsening. Our country discovered earlier that the prevention and control measures were appropriate and well controlled. Nucleic acid sequence analysis of the globally infected strains shows that the new coronary pneumonia is caused by a new type of coronary disease (SARS-CoV-2). SARS-CoV-2 is an RNA virus. The main mode of transmission is droplets and close contact. It is highly contagious. The genes of the virus strains recently discovered in Beijing Xinfa are the same as the European strains, indicating that under cold chain conditions, the virus survives longer and is more dangerous. Accurate and timely diagnosis and identification techniques are particularly important for the treatment of new coronary pneumonia and the control of the epidemic. Up to now, domestic and foreign mainly use nucleic acid and antibody detection methods, including reverse transcription-polymerase chain reaction (RT-PCR), gene sequencing, digital PCR, rapid detection of antibodies and antigens by colloidal gold technology, and magnetic particle chemiluminescence detection of antibodies Wait. This article describes the characteristics of different methodologies and the quality control of different methodologies for multi-dimensional analysis and detection of SARS-CoV-2. Provide a reference for the rapid and accurate diagnosis of new coronavirus SARS-CoV-2 and other bed infections.

Keywords： Biological Engineering SARS-CoV-2 fluorescent PCR method digital PCR technology gene sequencing colloidal gold method chemiluminescence method