介孔硅纳米粒的聚多巴胺修饰及药物负载的研究
首发时间:2020-08-14
摘要:目的:合成介孔硅纳米粒MSNs,经聚多巴胺PDA修饰后,负载化疗药物阿霉素DOX以及光热材料Cypate,最终制备得到载Cypate/DOX介孔硅纳米粒,并进行表征。方法:以CTAC为模板,在碱性条件下使硅源TEOS水解聚合形成MSN-OH,去除表面活性剂后,得到介孔硅纳米粒。经PDA修饰后,采用HEPES缓冲液法制备得到MSN-PDA,采用三乙胺脱盐法制备得到载DOX/Cypate介孔硅纳米粒MSN-PDA-Cypate/DOX。通过DLS和TEM考察纳米粒的尺寸大小,进行Zeta电位测定、紫外吸收检测、包封率和载药量的测定、药物体外释放、光热升温等实验,对其性质进一步考察。结果:介孔硅纳米粒TEM尺寸为24.85±0.14 nm,具有明显的介孔结构,Zeta电位表明纳米粒体系稳定。负载药物的MSN-PDA-Cypate/DOX纳米粒包封率为60.74%,载药量为7.79%(以DOX计算),该纳米粒呈现显著的光热升温能力和一定的靶向性释药。结论:成功合成并修饰介孔硅纳米粒,制得载Cypate/DOX介孔硅纳米粒。其稳定性较好,粒径均一,具有较高的药物负载能力和明显的光热升温效应。为肿瘤的化疗与光热协同治疗提供一种策略。
关键词: 药剂学 介孔硅纳米粒 聚多巴胺 阿霉素 Cypate 纳米药物
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The Study of Polydopamine Modified and Drug-loaded Mesoporous Silica Nanoparticles
Abstract:objective: To prepare mesoporous silica nanoparticles, modified by polydopaminne (PDA), loaded with doxorubicin (DOX) and Cypate. Methods: Mesoporous silica nanoparticles were synthesized by hydrolysis and polymerization of silica-derived TEOS in alkaline solution using CTAC as template. After being modified by PDA, MSN-PDA was prepared through HEPES buffer method, and MSN-PDA-Cypate/DOX was prepared through triethylamine desalting method. The characterization of morphology by DLS and TEM, Zeta potential measurement, UV absorption, encapsulation efficiency and drug loading tests, drug release, and photothermal effects were conducted, respectively. Results: The TEM size of the mesoporous silica nanoparticles was 24.85±0.14 nm, which had an obvius mesoporous structure. The Zeta potential indicated that the nanoparticle system was stable. MSN-PDA-Cypate/DOX nanoparticles had an encapsulation rate of 60.74% and drug loading of 7.79% (calculated based on DOX). Conclusion: We have successfully prepared MSN nanoparticles and MSN-PDA-Cypate/DOX nanopaticles. It was found that the stability and uniformity of particle size was good. Besides, the nanoparticles improved drug loading. It also exhibited a significant photothermal capacity and a certain targeted drug release. The research may provide an effective strategy for tumor treatment combined with chemotherapy and photothermal therapy.
Keywords: Pharmaceutics Mesoporous silica nanoparticles Polydopamine Doxorubicin Cypate Nanomedicine
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介孔硅纳米粒的聚多巴胺修饰及药物负载的研究
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