Effects of Selenium-enriched green tea on liver fibrosis induced by carbon tetrachloride in mice

• 1、School of Public Health, Soochow University,Suzhou 215123
• 2、School of Radiation Medicine and Protection, Soochow University, Suzhou 215123

Abstract：Objective: To investigate the effect of selenium-enriched green tea on liver fibrosis induced by carbon tetrachloride (CCl4) in male C57BL/6J mice. Methods: A total of 48 male C57BL/6 mice were randomly divided into four groups as follows: CON group, CCl4 group, CCl4+common tea group and CCl4+selenium-rich tea group, with 12 animals each group. Liver fibrosis was induced by intraperitoneal injection of 0.1 ml/100 g CCl4 (10% CCl4 in olive oil) twice per week. The mice in intervention groups drank tea and selenium-enriched tea solution for 4 weeks. At the end of the 28 days, the mice were deprived of food for 12 h and sacrificed. Results: CCl4 resulted in hepatic fibrosis and damaged hepatic function. Histological analysis of liver showed that tea interventions alleviated hepatic fibrosis and decreased hepatic collagen type I (COL1A1), collagen type III (COL3A1) and α-smooth muscle actin (α-SMA). Both tea interventions significantly increased serum levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and decreased tumor necrosis factor-α (TNF-α), transforming growth factor-beta1 (TGF-β1), interleukin-1β (IL-1β) and interleukin-6 (IL-6), malondialdehyde (MDA) content. Compated with in CCl4+common tea group, The improvemment was more obvious in CCl4+selenium-rich tea group. Conclusion: Both types of tea improve CCl4-induced liver fibrosis oxidative stress and inflammatory response in mice, with pronounced efffect in selenium-rich tea. ?????

Keywords： Food nutrition Hepatic fibrosis Selenium-rich green tea Oxidative stress