Characterization and isolation of estradiol degrading bacteria Acinetobacter sp. (7-1) Isolated from Changchun, China.
首发时间:2020-12-22
Abstract:17β- Estradiol, as a major environmental estrogen, is a serious threat to human health and ecological security. The MS medium containing 0.5 mM estradiol as the sole carbon source was used to isolate a new Gram-negative bacteria from the fishing pond. This screening strain can use as the sole carbon source and energy source such as estradiol and testosterone. After 16S rRNA analysis, the bacteria belong to Acinetobacter sp with 99.71% identity. The strain was Gram-negative bacilli, Spherical, Blunt round ends, Scattered or arranged in pairs, No bud, No flagella. Size 2.126μm×0.753μm; Conditions for optimum degradation of estradiol were 27℃, 150 rpm, and substrate concentration was 1 mM/L, pH7.5 and 1% inoculum. The highest degradation rate, the degradation rate can reach 85%. The strain could grow normally in 5 μg/mL auromycin, but not in 100 μg/mL ampicillin,50 μg/mL kanamycin,10 μg/mL streptomycin. The degradation rate of 17β- estradiol was 81.4%, that of testosterone was 64.6%, that of progesterone was 44.6%, and that of 17α- ethinyl estradiol was 62.3% by high performance liquid chromatography.
keywords: Biological detection Engineering 16s rDNA Estradiol degrading bacteria High performance liquid chromatography
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17β-雌二醇降解菌的筛选及鉴定
摘要:17β-雌二醇作为一种主要的环境雌激素对人类健康和生态安全具有严重的个威胁,近年来逐渐引起了国内外学者的广泛关注。17β-雌二醇具有作用强、危害广等特点,17β-雌二醇的毒害浓度相当低,只需1.0ng/L。因此深入研究其在环境中的作用机制及降解途径,对于控制和治理17β-雌二醇的污染具有重要意义。生物修复法即利用微生物降解环境雌激素是解决雌二醇污染的最有效方法之一。 本文采用含有0.5 mM雌二醇作为唯一碳源的MS培养基从渔塘中分离得到一种新型革兰氏阴性菌Acinetobacter sp,该筛选菌株可使用如雌二醇,睾丸酮等作为唯一碳源和能源。经16S rDNA分析显示该细菌属于莫拉菌科的不动杆菌属。该筛选菌株为革兰阴性杆菌,呈球杆状,两端钝圆,散在或成对排列,无芽胞,无鞭毛。尺寸大小为2.126 μm×0.753 μm;最佳降解条件为27℃,150rpm/min,底物浓度为1mM/L,pH值为7.5并且接种率为1%时降解率最高,降解率可达到85 %。该筛选菌株可在5ug/mL的金霉素可正常生长,而在100 μg/ml氨苄青霉素、50 μg/ml卡那霉素、10 μg/ml链霉素等中均不可生长。高效液相色谱法(HPlC)检测其对17β-雌二醇降解率为81.4%,对睾丸酮降解率为64.6%,对孕酮降解率为44.6 %,对17α-乙炔基雌二醇降解率为62.3%。 本文为类固醇激素降解菌的筛选已经对实际污水中的处理方式提供了理论依据和实验基础。
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