Preparation and Quality Analysis of Gardenia jasminoides Pectin

• 1、College of Pharmaceutical Sciences,Soochow University,Suzhou 215123

Abstract：Objective: To explore the extraction method of pectin (GJP) from Gardenia jasminoides Ellis, as well as its structure characterization and quality analysis. Methods: GJP was extracted from Gardenia jasminoides slag by thecombined acid-enzyme extraction.Its molecular weight was determined by HPSEC method, and the composition of monosaccharides was detected by HPEAC-PAD.Its structurewas analyzed by infrared, 1H and 13C NMR spectroscopy.Acid - alkali chemical titration method was used to determine the degree of esterification, the meta-hydroxybiphenyl method was used to determine the content of galacturonic acid, and the anthrone sulfuric acid method was used to determine the content of neutral sugars. Results: The relative molecular weight of GJP was 44.10 KDa, and it was composed of 7 monosaccharideinclude galacturonic acid, galactose, rhamnose, arabinose, xylose, mannose, glucose, and fucose. GJP was HG type pectin. The galacturonic acid content of GJP was measured to be 88.1%, neutral sugar content was 7.65%,. The degree of esterification was 72%, which indicated that it was high DE pectin. Conclusion: GJPcan be extracted from gardenia slag, which can be provided as a valuble by-product.

Keywords： pectin Gardenia jasminoides molecular weight monosaccharide composition content degree of esterification