Efficient synthesis and characterization of polysaccharide nano-selenium using hyperbranched polysaccharides from Pleurotus tuber-regium

• 1、School of Biotechnology, Jiangnan University, Wuxi 214122

Abstract：To improve the synthesis efficiency of polysaccharide nano-selenium and ensure its stability, a new method for the efficient synthesis of polysaccharide nano-selenium was developedusing hyperbranched polysaccharide from Pleurotus tuber-regium as raw material by comparing the common synthesis methods. The method used pre-acidified hyperbranched polysaccharide molecules as soft template and used in situ reduction to encapsulate stabilized nano-selenium.The optimum conditions for the synthesis of hyperbranched polysaccharide nano-selenium at 50℃ were obtained by single factor and response surface experiments: the molar concentration of Vc was 0.12 mol/L, the concentration of nitric acid was 0.1%, and the concentration of polysaccharide was 2.35 mg/L. The selenium content of producednanoparticles could be up to 394.65 mg/g with small particle size of 163.74 nm.The polysaccharide nano-sized selenium particles obtained by this method have higher selenium content and smaller particle size with good stability at the resolution concentration of 10 mg/mL.The results showed that hyperbranched polysaccharide was an ideal carrier for the synthesis of polysaccharide nano-selenium particles, which provided a new idea for the development of polysaccharide nano-selenium products.

Keywords： Pleurotus tuber-regium hyperbranched polysaccharides selenium nanoparticles particle size