High-density Cultivation of Recombinant Escherchia coli for Tyrosol

• 1、School of Biotechnology,Jiangnan University,Key Laboratory of Industrial Biotechnology ,Ministry of Education

Abstract：In order to further increase the tyrosol production of the high-yield tyrosol strains in our laboratory, the shake flask fermentation conditions were optimized by response surface method, and the fermentation expansion experiment was carried out through a 5L fermentor.The results of the response surface optimization shake flask are: the concentration of Ca2+ in the medium is 1.63 mmol/L, the ammonium sulfate is 3.28g/L, the temperature is 27℃, the fermentation time is 60h, and the tyrosol production is 2.80g/L, which is compared with the published recombinant large intestine.The level of tyrosol produced by the bacterium increased by 80%.The 5L fermenter was fed-batch fermentation, the fermentation time was 48h, and the OD600 was 36±0.5, which was an increase of about 10 compared to before optimization; the tyrosol yield was 5.06g/L, which was 74.5% higher than the shake flask yield, and the cell density and cell density.The output has increased significantly, laying the foundation for industrial production of tyrosol.

Keywords： High density fermentation Recombinant Escherichia coli tyrosol