Lactobacillus paragasseri FTJWQ2L9 relieves hyperuricemia in mice by increasing short-chain fatty acids production

• 1、School of Food Science and Technology, Jiangnan University, Wuxi 214122

Abstract：In order to explore the effect of Lactobacillus paragasseri on hyperuricemia, 30 male Kunming mice aged 5 weeks were selected for this study. During the experiment, 109 CFU of Lactobacillus paragasseri was administered daily, and potassium oxonate and hypoxanthine were daily administrated for the last 2 weeks to make mice hyperuricaemia. After the animal experiment, uric acid concentration in serum and urine, xanthine oxidase activity and the degree of kidney damage were measured, and the levels of short-chain fatty acids and changes in intestinal flora were analyzed. The results showed that Lactobacillus paragasseri FTJWQ2L9 can effectively reduce the concentration of uric acid in serum and urine of mice, inhibit the activity of xanthine oxidase (XOD) in the liver and serum, and restore the kidney damage in mice. In addition, Lactobacillus paragasseri FTJWQ2L9 could also increase the concentration of 6 short-chain fatty acids in mouse feces, and up-regulate the abundance of Ruminococcaceae UCG-005, Prevotellaceae NK3B31 group and Ruminococcaceae UCG-014. The results of correlation analysis between intestinal flora, short-chain fatty acid (SCFA) levels and uric acid metabolism index showed that uric acid and xanthine oxidase activities in serum had a strong correlation with short-chain fatty acid concentrations and SCFA-producing related taxa. Therefore, the regulation of Lactobacillus paragasseri FTJWQ2L9 on the level of SCFAs and its related taxa may be an important way for it to lower uric acid. This study provides a new idea for exploring the mechanism of probiotics to relieve hyperuricemia and provides a basis for follow-up research.

Keywords： Lactobacillus paragasseri hyperuricaemia short-chain fatty acids intestinal flora