印染染料高效降解菌剂的制备及应用
首发时间:2021-03-03
摘要:为获得规模化的染料高效降解菌剂培养技术,基于染料脱色效果对染料降解菌群进行发酵工艺优化,使其实现规模化培养及初步应用。通过GC-MS (Gas chromatography-mass spectrometer)确定菌群对污染物的降解能力;利用不同发酵罐对菌群进行发酵工艺优化及放大培养;将发酵液与载体复配得到菌剂并进行初步应用研究。结果表明,添加菌群培养液能大幅度提高污染物降解效率。在300 r/min、1.25 v v-1m-1及补料碳氮比为6:1的条件下菌群生长最佳,并且能够维持较高的染料脱色率。菌剂在保存四周后仍具有较高的染料脱色性能,连续脱色10个批次后脱色率仍能保持40%以上。在印染废水中添加菌剂处理能够明显改善废水性能并且加速COD及氨氮去除。该菌群具有较高的稳定性和染料脱色率且能够实现对印染废水处理性能的改善,为工业化应用奠定了基础。
关键词: 发酵工程 微生物菌群 染料降解 固态菌剂 功能稳定性 印染废水
For information in English, please click here
Preparation and application of high-efficiency decolorizing bacteria agent for dyes
Abstract:In order to obtain a large-scale cultivation technology of high-efficiency dye-degrading bacteria, the fermentation process of dye-degrading flora was optimized based on dye decolorization effect to realize large-scale cultivation and initial application. GC-MS (Gas chromatography-mass spectrometer) was used to determine the contaminants degradability by flora. The different fermentation tanks were used to optimize the fermentationPreparation and application of high-efficiency decolorizing bacteria agent for dyes process and scale up the culture of flora. The fermentation broth and the carrier were compounded to obtain the inoculum and initial applied research. The results showed that the addition of bacterial culture medium can greatly improve the pollutant degradation efficiency. Under the conditions of 300 r/min, 1.25 v v-1m-1 and the feed carbon to nitrogen ratio of 6:1, the flora grew best and can maintain a high dye decolorization ratio. The bacterial agent still has high dye decolorization performance after storage for four weeks, and the decolorization ratio can maintain over 40% after 10 batches of continuous decolorization. Adding bacterial agents to the dyeing wastewater treatment can significantly improve the performance of the wastewater, it also can accelerate COD and ammonia nitrogen removal. The flora has high stability and dye decolorization ratio, it can improve the dyeing wastewater treatment performance, laying the foundation for industrial application.
Keywords: Fermentation engineering Microbial flora Dye degradation Solid bacteria Functional stability Dyeing wastewater
基金:
引用
No.****
动态公开评议
共计0人参与
勘误表
印染染料高效降解菌剂的制备及应用
评论
全部评论0/1000