草地贪夜蛾两个性信息素结合蛋白的功能研究
首发时间:2021-04-16
摘要:性信息素结合蛋白(Pheromone binding proteins,PBPs)是鳞翅目昆虫中一类特异结合和转运性信息素的气味结合蛋白,在夜蛾科昆虫中主要存在于雄虫触角中。对PBPs基因的功能研究可为开发新型行为调节剂提供重要理论参考。本文对重要入侵害虫草地贪夜蛾的PBPs基因开展功能研究,首先通过电生理确定入侵的广东种群对雌蛾性信息素七种不同成分的生理活性,发现广东种群对Z9-14:Ac和Z7-12:Ac两种成分的触角电位变动明显。根据草地贪夜蛾基因组数据,利用生物信息学技术分析PBPs基因家族信息,结合荧光定量PCR筛选出在触角高表达的SFR10933和SFR21187作为候选基因开展功能研究。通过RNAi和电生理探究了两个基因的功能。本研究初步测定了草地贪夜蛾广东种群的雄虫对雌虫释放的性信息素的触角电位反应,为针对性诱捕广东草地贪夜蛾的性诱剂的改良提供了参考。同时,推测草地贪夜蛾触角中的PBPs基因在感受信息素时可能存在协同作用,在开发基于靶向PBPs的行为干扰剂时可考虑多靶向干扰。
关键词: 草地贪夜蛾 性信息素结合蛋白 基因表达分析 触角电位反应
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Functional characterization of two sex pheromone binding proteins in Spodoptera frugiperda
Abstract:Sex pheromone binding proteins (PBPs) are a type of odor binding protein that specifically binds and transports sex pheromones in Lepidopteran insects. They are mainly found in the antennae of males in Noctuidae. The functional study of PBPs genes can provide important theoretical references for the development of new behavior regulators. In this paper, a functional study of the PBPs genes of the important invasive pest Spodoptera frugiperda was carried out. First, the physiological activity of the invaded Guangdong population on the seven different components of female moth sex pheromone was determined by electrophysiology, and it was found that the Guangdong population was resistant to Z9-14:Ac and Z7-12:Ac The antenna potential of the two components of Ac changes significantly. According to the genomic data of Spodoptera frugiperda, bioinformatics technology was used to analyze PBPs gene family information, combined with fluorescent quantitative PCR to screen out SFR10933 and SFR21187 highly expressed in antennae as candidate genes for functional research. The functions of the two genes were explored through RNAi and electrophysiology. This study preliminarily determined the antennal potential response of the males of the Guangdong population of Spodoptera frugiperda to the sex pheromone released by females, and provided a reference for the improvement of sex attractants for the targeted trapping of Spodoptera frugiperda. At the same time, it is speculated that the PBPs genes in the antennae of Spodoptera frugiperda may have a synergistic effect in sensing pheromones, and multi-target interference can be considered when developing behavioral interference agents based on targeted PBPs.
Keywords: Spodoptera frugiperda sex pheromone binding protein gene expression analysis electroantennogram response
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