YAP参与了牙髓炎症及炎症损伤后的修复过程
首发时间:2021-04-23
摘要:背景及目的:牙髓炎症是影响牙髓修复的关键,轻微的炎症或者及时去除刺激,有利于牙髓的愈合;持续的炎症,使牙髓修复能力减弱甚至引起牙髓坏死。YAP是一种与细胞的矿化过程有关的转录共激活因子,不仅参与调节炎症过程还与多种细胞的矿化过程有关。但是,YAP是否参与牙髓炎症及炎症损伤后的修复过程尚不清楚。因此,本课题探究了YAP是否参与牙髓炎症损失及修复过程。方法:建立大鼠牙髓炎模型;收取人的健康牙髓和牙髓炎组织,免疫组织化学染色检测牙髓组织中YAP的表达。10 ng/mL TNF-α体外诱导牙髓细胞的炎症反应。qPCR检测mRNA的表达,Western blot检测蛋白的表达,茜素红S染色检测牙髓细胞的矿化能力。结果:牙髓炎组织中YAP的表达明显上调,TNF-α也上调牙髓细胞中YAP 的表达。诱导牙髓细胞矿化前,TNF-α预处理0.5小时促进了矿化,TNF-α预处理3小时和TNF-α持续刺激抑制了矿化。与对照组相比,矿化诱导液组中牙髓细胞的YAP蛋白表达上调。与矿化诱导液组相比,TNF-α预处理0.5小时抑制了YAP的表达;然而,TNF-α预处理3小时和TNF-α持续刺激上调了牙髓细胞YAP蛋白的表达。结论:YAP参与了牙髓炎症过程。短时间的炎症刺激促进了牙髓细胞的矿化,长时间炎症刺激抑制矿化。YAP参与了正常条件下牙髓细胞的矿化过程和受到炎症刺激的牙髓细胞的矿化过程。
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YAP IS INVOLVED IN THE PULP INFLAMMATION PROCESS AND INFLAMMATION-INDUCED REPAIR PROCESS
Abstract:Background and purpose: Pulp inflammation is the key to pulp restoration. Slight inflammation or timely removal of the irritation is beneficial to the healing of the pulp; continuous inflammation weakens the repair ability of the pulp and even causes pulp necrosis. YAP is a transcriptional co-activator related to the mineralization process of cells. It is not only involved in regulating the inflammatory process but also related to the mineralization process of a variety of cells. However, it is not clear whether YAP is involved in pulp inflammation and repair process after inflammation injury. Therefore, this topic explored whether YAP is involved in the pulp inflammation loss and repair process. Methods: The rat pulpitis model was established; human healthy pulp and pulpitis tissues were collected, and the expression of YAP in the pulp tissues was detected by immunohistochemical staining. 10 ng/mL TNF-α induced the inflammatory response of dental pulp cells in vitro. qPCR was used to detect mRNA expression, Western blot was used to detect protein expression, and Alizarin Red S staining was used to detect the mineralization ability of dental pulp cells. Results: The expression of YAP in pulpitis tissue was significantly up-regulated, and TNF-α also up-regulated the expression of YAP in dental pulp cells. Before inducing the mineralization of dental pulp cells, TNF-α pretreatment for 0.5 hours promoted mineralization, but TNF-α pretreatment for 3 hours and continuous TNF-α stimulation inhibited mineralization. Compared with the control group, the YAP protein expression of dental pulp cells in the mineralization induction liquid group was up-regulated. Compared with the mineralization induction liquid group, TNF-α pretreatment for 0.5 hours inhibited the expression of YAP; however, TNF-α pretreatment for 3 hours and continuous stimulation of TNF-α increased the expression of YAP protein in dental pulp cells. Conclusion: YAP is involved in the process of pulp inflammation. Short-term inflammatory stimulation promotes the mineralization of dental pulp cells, and long-term inflammatory stimulation inhibits mineralization. YAP participates in the mineralization process of dental pulp cells under normal conditions and the mineralization process of dental pulp cells stimulated by inflammation.
Keywords: endodontics YAP TNF-α pulpitis mineralization
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