Mechanism of Phellinus Igniarius Improving Iipid Metabolism Based on Network Pharmacology

• 1、College of Food Science and Engineering, Jilin University, Changchun 130062

Abstract：Background: Phellinus igniarius has very high medicinal value and existing studies have shown that it has a positive effect Study and Validation of Phellinus Igniarius to Improve Lipid Metabolism based on Network Pharmacologyon improving lipid metabolism, but the mechanism of action remains unclear. Objective: To explore the mechanism of Phellinus igniarius improving lipid metabolism based on network pharmacology. Methods: The active components of Phellinus igniarius were collected through literature review and database of traditional Chinese medicine and chemical constituents, and screened by SwissADME. The selected components were imported into pharmmapper database to obtain the potential targets of active componentsof Phellinus igniarius. In genecards, OMIM, drugbankand other databases, "hyperlipidemia" and "disturbed lipid metabolism" were used as keywords to obtain potential targets. Based on the intersection of potential targets of active ingredients and potential targets, the common targets of drugs and diseases were obtained. Based on that, the network of "Phellinus igniarius - active ingredients - common targets" was firstly visualized by using Cytoscape, and then the protein interaction network was generated by using STRING database. Finally, the GO function enrichment analysis and KEGG pathway enrichment analysis were carried out by using Metascape database to explore Phellinus igniarius improving the mechanism of lipid metabolism. Results: A total of 19 active components were screened from Phellinus igniarius, involving 143 targets. 3212 targets were found from "hyperlipidemia" and "distorted lipid metabolism". Among them, there are 93 targets with the same drug and disease, including ANG, APCs, BCHE, etc. The network of "Phellinus igniarius - active ingredients - common targets" includes one drug, 19 active ingredients, 93 targets, of which 10 important active ingredients were selected, including m-hemipinic acid, asparagic acid, sakuranetin, etc. Through network topology and protein interaction network analysis, 30 core targets were screened, including REN, GSTP1, TPI1, etc. By GO enrichment analysis, 911 biological process items (BP), 96 cell composition items (CC) and 35 molecular function items (MF) were obtained. KEGG pathway enrichment analysis screened 101 related signaling pathways, including PI3K Akt signaling pathway, endocrine resistance, insulin resistance and so on. Conclusion: The active components of Phellinus igniarius of this project may affect 15 pathways such as PI3K-Akt signaling pathway and PPAR signaling pathway by acting on PPARG, SOD2, RXRA, ALB, MAPK8 and so on, and play a regulatory role in improving lipid metabolism. This project may explain complex network relations by the method of network pharmacology. The network pharmacology research results can be combined with conventional experiments to better help improve the understanding of improving lipid metabolism mechanism, from the molecular level and provide significant theoretical basis on research directions such as improving lipid metabolism disorder and treating hyperlipidemia as well as drug development.

Keywords： Pharmacology；Network pharmacology；Phellinus igniarius；Lipid metabolism；Signaling pathways