Construction of inflammatory stress IDILI model based on microfluidics

• 1、College of Pharmaceutical Sciences,Soochow University,Suzhou,215123

Abstract：Idiosyncratic Drug-Induced Liver Injury (IDILI) is the main pathogeny of clinical hepatotoxicity in post-marketing surveillance. It has low incidence, but may cause serious consequence such as liver failure. The mechanism has not yet been clarified. Inflammatory stress is one of the important hypotheses of idiosyncratic drug-induced liver injury. At present, it mainly relies on low-dose LPS animal models for characterization. However, due to different drug, the parameters of animal models often change, and hepatic inflammation situation can not be detected promptly. In this paper, based on flexible microfluidic technology, a laminated liver chip containing HepG2, U937 macrophages, LX-2, EA.hy926 cells and an inflammation model were constructed, and troglitazone, the known IDILI drug, was evaluated. This chip is a powerful tool with friendly operation and high throughput, and can be used to parallelly evaluate apparent activity of cells under different conditions. With the chip, we can get better understanding of inflammatory stress drug-induced liver injury.

Keywords： Microfluidic Chip Inflammatory stress Idiosyncratic drug-induced liver injury Lipopolysaccharide Troglitazone