基于四细胞肝器官芯片的地塞米松肝损伤评价
首发时间:2022-11-03
摘要:食品中添加剂的安全性评价是食品安全研究的重点之一。肝脏作为内、外源物质发生生物转化的主要场所,是重要的毒靶器官。本文基于微流控技术构建了包含肝实质和非实质细胞的仿生肝血窦芯片,评估了不同仿生条件下、不同剂量浓度地塞米松的肝损伤程度。研究结果表明,相对于孔板实验,仿生肝血窦芯片内肝细胞毒性损伤更弱,且给出了地塞米松作用于不同肝细胞的综合表观损伤结果,更符合体内的药物作用过程。本研究表明微流控肝芯片可以成为食品添加剂安全评价的有力工具。
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Evaluation of dexamethasone liver injury based on four-cell liver organ chip
Abstract:The safety evaluation of additives in food is one of the focuses of food safety research. As the main site of biotransformation of endogenous and exogenous substances, liver is an important toxic target organ. In this paper, based on microfluidic technology, a bionic liver sinusoid chip containing liver parenchymal cells and non-parenchymal cells was constructed to evaluate the degree of liver injury caused by dexamethasone at different concentrations under different bionic conditions. The results showed that compared with the plate experiment, the hepatocyte toxic damage in the bionic liver sinusoid chip was weaker, and the comprehensive apparent damage results of dexamethasone on different hepatocytes was given, which were more consistent with the drug action process in the body. This study shows that microfluidic liver chips can become a powerful tool for the safety evaluation of food additives.
Keywords: organ-on-chip safety evaluation Liver injury In vitro model.
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