小麦蛋白激酶基因TaSnRK2.4介导植株抵御干旱功能研究

李天娇; 肖凯

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小麦蛋白激酶基因TaSnRK2.4介导植株抵御干旱功能研究

首发时间：2022-12-30

李天娇 ¹
李天娇（1998-），女，全日制硕士研究生，主要研究方向：作物栽培学与耕作学
肖凯 ¹
肖凯（1963-），男，博导，主要研究方向：作物抗逆与分子生物学

1、河北农业大学农学院，保定市，701001

摘要：蔗糖非酵解蛋白激酶家族2 (Sucrose non-fermenting-1-related protein kinase 2，SnRK2)成员通过介导环境信号传递，在调控植株响应和抵御非生物逆境过程中发挥着重要作用。本文以前期鉴定的1个小麦种属SnRK2家族成员TaSnRK2.4为基础，对该基因分子特征、应答干旱胁迫表达模式及介导植株抵御干旱逆境的功能进行了研究。结果表明，TaSnRK2.4编码蛋白含有含有激酶保守的Ser/Thr保守结构域，在氨基酸序列及核苷酸水平上与植物种属中部分SnRK2家族成员高度相似。植株根、叶中TaSnRK2.4转录本均呈明显干旱诱导表达模式。与野生型（WT）相比，干旱处理下，正义表达TaSnRK2.4烟草株系（Sen 1）长势增强，干鲜重增加，气孔关闭速率加快，光合能力增强，细胞保护酶活性提高，可溶性蛋白和可溶性糖含量增加；反义株系（Anti 1）长势则较WT减弱，气孔关闭速率减慢，上述干旱相关生理参数较WT变差。研究表明，TaSnRK2.4通过在转录水平上对干旱胁迫产生应答，对植株干旱适应相关生理生化过程进行正向调节，在增强植株抵御干旱胁迫能力中发挥重要作用。

关键词：小麦蛋白激酶 TaSnRK2.4 干旱胁迫遗传转化功能分析

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Functional Characterization of a Wheat Protein Kinase Gene TaSnRK2.4 in Mediating Plant Tolerance to Drought Stress

LiTianjiao ¹
李天娇（1998-），女，全日制硕士研究生，主要研究方向：作物栽培学与耕作学
XiaoKai ¹
肖凯（1963-），男，博导，主要研究方向：作物抗逆与分子生物学

1、College of Agronomy, Hebei Agricultural University, Baoding city 071001

Abstract：Members of the sucrose non-ferment-1-related protein kinase 2 (SnRK2) family play an important role in plant response and resistance to abiotic stresses by mediating environmental signaling. Based on TaSnRK2.4, a member of the SnRK2 family of wheat species previously identified, the molecular characteristics of this gene, its expression pattern in response to drought stress, and its function in mediating plant resistance to drought stress were investigated. The results showed that the TaSnRK2.4 protein contains Ser/Thr conserved domains as generally found in kinases. At nucleic acid and amino acid levels, this wheat SnRK2 member display high similarities to its counterparts in diverse plant species. Expression analysis revealved that the transcripts abundance of TaSnRK2.4 was induced in both roots and leaves under drought stress conditions. Transgene analysis revealed that compared with wild-type (WT), under drought treatment, the tobacco line with sense expression of TaSnRK2.4 (Sen 1) displayed enhanced growth feature and increased plant fresh weight and biomass, promoted stomatal closing rate, elevated photosynthetic capacity, enhanced cellular protective enzyme activities, and increased soluble protein and sugar contents. In contrast, the line with anti-sense expression of TaSnRK2.4 (Anti 1) exhibited alleviated growth phenotype and lowered dry and fresh weights, slowed stomatal closing rate, and worsened above drought-related physiological parameters compared with WT under drought condition. Our results suggested that TaSnRK2.4 is response to drought signaling at transcriptional level, positively regulating the physiological and biochemical processes related to drought adaptation of plants and plays an important role in enhancing the ability of plants to resist the drought stress.

Keywords： Wheat Protein kinase, TaSnRK2.4 Drought stress Genetic transformation Functional analysis

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李天娇，肖凯. 小麦蛋白激酶基因TaSnRK2.4介导植株抵御干旱功能研究[EB/OL]. 北京：中国科技论文在线 [2022-12-30]. https://www.paper.edu.cn/releasepaper/content/202212-110.

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论文编号	202212-110
论文题目	小麦蛋白激酶基因TaSnRK2.4介导植株抵御干旱功能研究
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