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2003-2020 全部
为您找到包含“分析化学”的内容共187

JIANG Han-Peng,CHU Jie-Mei,LAN Meng-Dan,LIU Ping,YANG Na,ZHENG Fang,FENG Yu-Qi,YUAN Bi-Feng

More than 140 modified nucleosides have been identified in RNA. Determination of endogenous modified nucleosides in biological fluids may serve as non-invasive disease diagnostic strategy. However, detection of the modified nucleosides in biological fluids is challenging, especially for the low abundant modified nucleosides due to the serious matrix interferences of biological fluids. Here, we developed a facile preparation strategy and successfully synthesized zirconium oxide-silica (ZrO2/SiO2) composite capillary monolithic column that exhibited excellent performance for the selective enrichment of cis-diol-containing compounds. Compared with the boronate-based affinity monolith, the ZrO2/SiO2 monolith showed ~ 2 orders of magnitude higher extraction capacity and can be used under physiological pH (pH 6.5-7.5). Using the prepared ZrO2/SiO2 composite monolith, we further established an online solid-phase microextraction (SPME) in combination with liquid chromatography-mass spectrometry (online SPME-LC-MS/MS) analysis for the comprehensive profiling nucleosides modification in human urine. Our results showed that 68 cis-diol-containing nucleosides and ribosylated compounds were identified in human urine, which is, to the best of our knowledge, the most numbers of cis-diol-containing compounds were determined in a single analysis. It is worth noting that four modified nucleosides were discovered in the human urine for the first time. In addition, the quantification results showed that compared to healthy controls, 16, 11 and 7 ribose conjugates increased more than 2 folds in the urine of gastric cancer, esophagus cancer and lymphoma, respectively. These increased ribose conjugates in cancer patients suggested that the metabolism of these compounds may have important roles in carcinogenesis as well as in serving as indicator of cancers.

2016-05-27

National Natural Science Foundation of China(21522507

Ph.D. Programs Foundation of Ministry of Education of China( 21475098

National Basic Research Program of China (973 Program) (20120141120037

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China.,Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China.,Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China.,Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China.,Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.,Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.,Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 43007

#Chemistry#

0评论(0 分享(0)

JIANG Han-Peng,XIONG Jun,HUANG Wei,LAN Meng-Dan,ZENG Huan,FENG Yu-Qi,YUAN Bi-Feng

Aging is characterized by progressive decline in physiology and function of adult tissues. Previous studies demonstrated DNA methylation is related to aging. In addition to DNA methylation, reversible RNA modification has recently been proposed to represent another realm for biological regulation in the form of "RNA epigenetics". However, the correlation of emerging RNA epigenetic modifications in aging process is still a mystery. Here we developed an on-line trapping/capillary hydrophilic-interaction liquid chromatography/electrospray ionization-mass spectrometry method for ultra-sensitive and simultaneous quantification of RNA epigenetic modifications of N6-methyladenosine (m6A) and 5-methylcytosine (5-mC) from human blood. We analyzed the contents of m6A and 5-mC in RNA of blood from 260 healthy persons aged from 0 to 88 years old. The results showed that both m6A and 5-mC contents were significantly decreased and highly correlated with aging process. We then established RNA epigenetic modifications age models according to m6A and 5-mC contents with an mean absolute deviation of 6.7 years for m6A and 8.0 years for 5-mC. This study demonstrated RNA epigenetic modifications of m6A and 5-mC represents strong and reproducible marks of chronological age, which could be potentially applied in health assessment and prevention of diseases.

2016-05-27

National Natural Science Foundation of China(21522507

National Basic Research Program of China (973 Program)( 21475098

Ph.D. Programs Foundation of Ministry of Education of China(2012CB720601

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China,Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China,Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China,Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China,Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China,Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, Chin

#Chemistry#

0评论(0 分享(0)

刘璇,雷建平

2011-02-18

量子点材料以其独特的物理和化学性质,已被广泛地在生物分析中应用。由于电致化学发光(ECL)技术集成了发光分析高灵敏度和电化学电位可控性的优点,极大地吸引了分析化学工作者的兴趣。开发水溶性量子点ECL

高等学校博士学科点专项科研基金新教师计划(20070284052

南京大学化学化工学院,南京大学化学化工学院,南京210093

#化学#

0评论(0 分享(0)

陈石,何华,张志强

2007-11-06

利用分子力学MM+和量子力学PM3方法对一系列7-(N-取代苯乙基)-哌嗪-6-氟喹诺酮类化合物分子进行计算, 并由所计算的结构参数结合与活性指标进行多元逐步回归处理, 建立了相关性较好的构效关系方程式,并对影响抗菌活性的主要因素作了讨论

中国药科大学分析化学教研室,中国药科大学分析化学教研室,中国药科大学分析化学教研室

#药学#

本文收录在《中国科技论文在线精品论文》收录于2008年6月第1卷第3期

0评论(0 分享(0)

孙宝珠

2011-01-12

采用水洗、酸洗、碱洗、热处理等方法对样品进行处理,然后对处理好的样品进行X射线衍射测试与X射线荧光测试。分析得知样品中最主要的物质是Mn(OH)4和K2-xMn8O16,主要的杂质元素是S元素和K元素。经过水洗处理能有效的除去杂质元素S,对K的去除效果并不理想。经过酸洗结合水洗后物质的结构没有变化。经过热处理结合酸洗、碱洗、水洗处理后, Mn(OH)4发生分解生成了MnO2, K2-xMn8O16未发生变化。

中国矿业大学材料科学与工程学院

#化学#

0评论(0 分享(0)

裘兰兰,何华

2007-07-17

手性是生物聚合物的特征性质,而树状大分子可作为一种天然高分子的模型化合物,在树状大分子上并入手性基团后可得到一类具有特殊光学性质的化合物-手性树状大分子。本文对手性树状大分子的制备,性质和应用做了扼要的介绍,并探讨了此类大分子在该领域中的研究和应用前景。

中国药科大学基础部分析化学教研室,中国药科大学基础部分析化学教研室

#化学#

0评论(0 分享(0)

李广伟,孙建军

2011-02-25

本文成功制作了ITO膜热电极,并对该电极的电化学和温度等性质进行了表征。研究了曝光时间、显影条件对电极刻蚀条件的影响以及电极形状对加热效果的影响。结果表明,电极温度的升高与加热电流的平方成正比;与常规的柱电极不同,在相同的加热电流下,电极的放置方式(垂直或水平)对加热效果几乎不产生影响。电极温度可在0.1s时间内快速升高和降低并达到平稳值;在长时间连续加热过程中(连续3600s),该电极表现出了对温度良好的热稳定性。

教育部博士点基金(20070386005

国家自然科学基金(20775015

福州大学化学化工学院,福州大学化学化工学院

#化学#

0评论(0 分享(0)

YANG Ya,PAN Jinyin,CHEN Yun,TU Yifeng

In this paper, an immunosensor based on self-assembly modified (SAM) Au electrode for the specific detection of morphine (MO) is reported. By the strategy of 3-mercaptopropionic acid SAM, activated by 1-ethyl-3-(3-dimethylaminoprophyl) carbodiimide (EDC) / n-hydroxysulfsuccinimide (NHS) and the immobilization of abundant morphine antibody, the proposed immunosensor provides the sensing signal based on its specific covalent binding with mophine. The technique of electrochemical impedance spectroscopy (EIS) is used as the read-out signal. The value of electron transfer resistance (Ret) responds upon the concentration of mophine with a detection limit of 0.266 pg/L. The immunosensor can be regenerated for at least 2 times by the elution with NaOH-H3PO4 (0.1 mol/L, pH=13.0). When it was used to detect the MO in spiked serum samples, there were satisfactory recoveries between 84.1% and 110.9%. In conclusion, the proposed sensor is proven to be more advantageous compared to those once reported methods in terms of the sensitivity and simplicity.

2013-03-14

PhD Programs Fundation of Ministry of Education of China (20093201110004

Department of Forensic Science, Soochow University, JiangSu Suzhou 215123,Institute of Analytical Chemistry, Soochow University, Suzhou, 215123,Institute of Analytical Chemistry, Soochow University, Suzhou, 215123,Institute of Analytical Chemistry, Soochow University, Suzhou, 215123

#Chemistry#

0评论(0 分享(0)

李萌甜,侯贤灯

2017-04-27

汞及其化合物广泛存在于各类环境介质和食物链中,因其毒性以及生物放大作用,对环境和人类健康存在极大危害,因此对汞的检测及形态分析十分重要。本文概述了2012年至今,原子光谱法检测环境及生物样品中的汞,并从样品处理、进样方式、检测仪器以及分离方法方面进行了小结。

教育部博士点基金项目(编号:20120181110046

四川大学化学学院,成都 610064,四川大学分析测试中心,成都 610064

#化学#

0评论(0 分享(0)

王康,曹雷

2014-01-14

制备了金纳米粒子并对其进行DN修饰过程初步研究。利用暗场显微成像技术对分散于玻璃片表面的金纳米颗粒进行了成像,并观察了单个金纳米颗粒的散射光谱。为此我们还开发了一种将金纳米粒子固定在到载玻片表面的新方法,为后续的研究提供了一定帮助。

教育部博士点基金(20100091120033

南京大学化学化工学院,南京 210093,南京大学化学化工学院,南京 210093

#化学#

0评论(0 分享(0)