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2003-2018 全部
为您找到包含“牛血清白蛋白”的内容共54

Xuyang Liu,Zhihong Shi,Hanwen Sun

In this paper, the interaction of lomefloxacin (LMF) and/or cefazolin (CFZ) with bovine serum albumin (BSA) was studied by fluorescence quenching in combination with UV-Vis spectroscopic method under simulative physiological conditions. The fluorescence quenching constants, binding distance, and binding constants for BSA-LMX and/or CFZ systems were determined. The fluorescence quenching of BSA by addition of LMF and/or CFZ is due to static quenching and energy transfer. The ratio of binding constants (KA) for BSA-LMF to BSA-CFZ equals to 56.8. In the presence of CFZ (LMF), the binding distance of BSA-LMF (BSA-CFZ) decreased from 4.44 to 2.45 nm (from 1.94 to 1.48 nm), and the binding constant (KA) of BSA-LMF (BSA-CFZ) increased from 2.87×105 to 5.43×105 L/mol (from 5.15×103 to 1.24×104 L/mol). Two-coexisting CFZ and LMF may lead to the need for more doses to achieve therapeutic effect. Circular dichroism spectra, synchronous fluorescence, and three-dimensional fluorescence studies showed that the presence of LMF or CFZ could change the conformation of BSA during the binding process, and the presence of coexisting LMF and CFZ could change the conformation of BSA further, here LMF was reigning.

2014-01-23

Natural Science Found of Hebei Province (B2008000583) and the sustentation Plan of Science and Technology of Hebei Province

College of Chemical and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P. R. China.,College of Chemical and Environmental Science, Hebei University

#Chemistry#

张婷婷,张蓉,周艳晖,陈小佳,刘杰

2011-06-15

(p-mopip)]2+ (△-1)与血清白蛋白(BSA)之间的相互作用。荧光光谱表明钌配合物∧-1、1和△-1对BSA的荧光猝灭机制为静态猝灭。∧-1、1和△-1与BSA的结合常数分别为6.72×103

暨南大学化学系,暨南大学化学系,广州,510632,暨南大学化学系,广州,510632,暨南大学生物工程研究所,广州,510632,暨南大学化学系,广州,510632

#化学#

周小凤,何志朋,黄鹤

2015-12-10

采用原位红外光谱分析方法研究了温度扰动诱导水(H2O)溶液及重水(D2O)溶液中血清白蛋白(BSA)二级结构的变化过程。无论BSA在H2O还是D2O溶液中,各二级结构含量随温度变化的规律是基本

高等教育博士点基金(20113201110004

国家自然科学基金(51373115

苏州大学材料与化学化工学部,苏州,215123,苏州大学材料与化学化工学部,苏州,215123,苏州大学材料与化学化工学部,苏州,215123;苏州大学张家港工业技术研究院,张家港 215600

#化学#

Zhu Ling,Liu Xiaoyan,Zhang Haixia

Interaction of tamoxifen citrate (TC) with salmon sperm DNA and bovine serum albumin (BSA) were investigated by various spectroscopic analysis methods under simulative physiological conditions. Thermodynamic parameters showed that the interaction process was spontaneous and hydrophobic forces play a major role in the binding between BSA and TC. The quenching constant KSV, the bimolecular quenching constant kq and the numbers of binding sites n (about 1) of both DNA and BSA to TC were similar. The value of n approximately equal to 1 indicated that there is only one type of binding site for DNA or BSA to TC. However, the binding constant (K) of DNA to TC was lower than that of BSA.

2010-07-26

The Program for New Century Excellent Talents in University (NCET-07-0400

The National Natural Science Foundation of China Fund (No.20775029

State Key Laboratory of Applied Organic Chemistry, Lanzhou University,State Key Laboratory of Applied Organic Chemistry, Lanzhou University,State Key Laboratory of Applied Organic Chemistry, Lanzhou University

#Chemistry#

本文收录在《中国科技论文在线》,2010,5(12):950-954.

何凤群,曾成鸣

2010-02-11

近年来的研究发现,大约20多种人类疾病与蛋白质或多肽的淀粉样纤维化有关。尽管已经进行了大量的研究,但蛋白质的纤维化及其细胞毒性的分子机制仍然不清楚。血清白蛋白(BSA)是一种非致病蛋白质,本文对其

陕西师范大学生命科学学院,陕西师范大学化学与材料科学学院

#生物学#

Hui Lin,Jingfeng Lan,Min Guan,Fenling Sheng,Haixia Zhang

The mechanism of interaction between mangiferin (MA) and bovine serum albumin (BSA) in aqueous solution was investigated by fluorescence spectra, synchronous fluorescence spectra, absorbance spectra and Fourier transform infrared (FT-IR) spectroscopy. The binding constants and binding sites of MA to BSA at different reaction time were calculated and the distance between the MA and BSA was estimated to be 5.20 nm based on Föster’s theory. In addition, synchronous fluorescence and FT-IR measurements revealed that the secondary structures of the protein changed by the interaction of MA with BSA. Furthermore, the influence of β-cyclodextrin on the system was studied. As a conclusion, the interaction between the anti-diabetes Chinese medicine MA and BSA was studied preliminary which may provide some significant information for the mechanism of the chinese traditional medicine MA on the protein level to cure diabetes or other diseases.

2009-01-08

国家自然科学基金(20775029

新世纪人才计划(0

兰州大学青年骨干教师计划(0

College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, China,College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, China,College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, China,College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, China,College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, China

#Chemistry#

0评论(0 分享(0)

Fei Geng,Liqiang Zheng,Li Yu,Jie Liu,Zhen Li

The interactions between a surface active imidazolium ionic liquid (IL), 1-tetradecyl-3-methylimidazolium bromide (C14mimBr) and bovine serum albumin (BSA) were investigated by surface tension, isothermal titration microcalorimetry, far-UV circular dichroism (CD) and fluorescence spectra. The surface tension measurement shows the formation of C14mimBr/BSA complex and the effect of the complex on surface tension. The enthalpy change in the whole interaction process between C14mimBr and BSA was obtained by isothermal titration microcalorimetry, and the results show alteration of the BSA structure. The contents of α-helix and random coil were obtained by far-UV CD. Changes of these contents reveal that the secondary structure of BSA changes with addition of C14mimBr. Fluorescence spectra show that tryptophan (Trp) residues, one of the intrinsic fluorophores in BSA, are exposed to a hydrophobic microenvironment with the addition of C14mimBr.

2009-02-19

国家自然科学基金(20773081

山东省自然科学基金(重点)(Z2007B06

教育部博士点基金(新教师基金)(070422047

Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education,Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education,Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education,Department of Chemistry, Liaocheng University,Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education

#Chemistry#

徐敏,王艺峰,洪群峰

2009-12-16

本工作将层层自组装与分子印迹技术相结合,首先利用表面层层自组装技术,将血清白蛋白和壳聚糖固定在交联壳聚糖基材表面,再经环氧氯丙烷处理后,洗脱模板蛋白质,制备出血清白蛋白分子印迹聚合物。通过

国家自然科学基金(50703030

武汉理工大学材料科学与工程学院,武汉理工大学材料科学与工程学院,武汉理工大学材料科学与工程学院

#材料科学#

0评论(0 分享(0)

刘晓楠,石贤爱,王航,郭养浩,孟春

2007-05-06

本工作开发了一在平衡过程和电泳过程采用不连续缓冲体系进行样品毛细管电泳分析的技术。当采用低浓度的磷酸盐缓冲液平衡毛细管,高浓度的磷酸盐缓冲液电泳分离血清白蛋白(BSA)时,在电泳过程中可以浓缩

福州大学,福州大学生物科学与工程学院,福州大学生物科学与工程学院,福州大学生物科学与工程学院,福州大学生物科学与工程学院

#化学#

本文收录在《中国科技论文在线》收录于2007年12月第2卷第12期

0评论(0 分享(0)

时晓磊,金永日,李绪文,殷涌光

2014-08-20

山奈甲黄素-7-O-4"-O-乙酰基-鼠李糖基)-3-O-芸香糖苷(A3)与血清白蛋白之间的相互作用时,共存离子存在的条件下,离子对反应的协同作用,研究表明,在295K的条件下,Ca2+和Fe3+对

教育部博士点新教师基金(20120061120116

吉林大学军需科技学院食品质量与安全系,吉林大学化学学院分析化学系,吉林大学化学学院分析化学系,吉林大学生物与农业工程学院

#化学#