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2003-2020 全部
为您找到包含“神经干细胞”的内容共57

Liu Li,Lin Zhiguo

OBJECTIVE: To model the microenvironment in vivo, and to co-culture the NSCs with normal hippocampal neuron and "epileptic neuron" for the observation of NSCs development. DESIGN: Repeated measurement and observation. METHODS: Rat hippocampal neurons were isolated and cultured, magnesium-free media treatment was applied to establish the model of "epileptic neuron". NSCs were cultured according to regular methods. After labeled by green fluorescence protein, NSCs were co-cultured with normal hippocampal neuron or "epileptic neuron" for 14 days, respectively. Patch clamp was used to record the electrophysiology of NSCs in co-culture; immunocytochemistry was used to demonstrate the expression of synaptophysin antibody of NSCs; patch clamp was also used to record the postsynaptic potential of the neurons differentiated from NSCs in magnesium-free medium. RESULTS: After NSCs was co-cultured with normal hippocampal neuron, 14 beats/5 minutes excitatory postsynaptic potential was recorded in 60% NSCs (6/10) by patch clamp; After co-culture with "epileptic neuron", 12 beats/5 minutes excitatory postsynaptic potential of NSCs was recorded. Immunocytochemistry revealed that 80% NSCs (12/15) was observed to express the synaptophysin in co-culture with normal neuron or "epileptic neuron". In magnesium-free medium, 14 beats/5 minutes excitatory postsynaptic potential with a duration of about 10 seconds was found in 60% neurons differentiated from NSCs (9/15), and no epileptic discharge was recorded. CONCLUSION: Rat hippocampal NSCs can form functional synapse in the co-culture with "epileptic neuron" in vitro. The possibility that NSCs develop into "epileptic neuron" is minimal.

2011-02-06

Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China,Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China

#Clinical Medicine#

陈新林,刘勇,田玉梅

2009-02-11

本实验采用细胞培养和免疫细胞化学等方法,研究缺氧条件对大鼠脑神经干细胞(NSCs)增殖作用的影响。实验结果显示,应用MTT比色分析,与对照组比较,缺氧后增殖细胞数量显著增多(P<0.05),其增殖

高等学校博士学科点专项科研基金(20050698014

西安交通大学医学院,神经生物学研究所,环境与疾病相关基因教育部重点实验室,生理学国家重点学科,西安交通大学医学院,神经生物学研究所,环境与疾病相关基因教育部重点实验室,生理学国家重点学科,西安交通大学医学院神经生物学研究所

#生物学#

赵舒武,蔡青,王晓玲,郑纺

2012-09-27

目的:观察补阳还五汤载药血清对缺氧性环境中神经干细胞的增殖及其分化的影响。方法:从孕14 d(E14 d)大鼠获得神经干细胞,经无血清培养基悬浮培养并传代。取传3代神经干细胞,添加不同浓度的载药血清

教育部科技发展中心高校博士点基金资助课题(编号20091210120001

天津中医药大学中医学院,天津 300193,天津中医药大学中医学院,天津 300193,天津中医药大学中医学院,天津 300193,天津中医药大学中医学院,天津 300193

#基础医学#