2018-05-29
本工作设计并合成了一种新型的检测溶酶体内硫化氢的比率型荧光探针。该探针是以萘酰亚胺为荧光团，对叠氮基苄基为识别位点，N-（2-氨基乙基）吗啉为靶向基团设计的。在405 nm光激发下，该探针本身
Chemistry and Chemical Engineering School,Hunan University,Changsha City Post Code 410082;Chemistry and Chemical Engineering School,Hunan University,Changsha City Post Code 410082;Chemistry and Chemical Engineering School,Hunan University,Changsha City Post Code 410082,,
#化学#
2012-03-13
本文提出了一种新的检测痕量Bi(III)的荧光方法。该法利用Bi(III)淬灭Tb荧光探针荧光的原理检测Bi(III)，Bi(III)浓度在0-30 pM范围内，探针的荧光强度与Bi(III)浓度
东南大学生物科学与医学工程学院，生物电子学国家重点实验室，南京，210096,东南大学生物科学与医学工程学院，生物电子学国家重点实验室，南京，210096
#化学#
2019-05-21
本文设计合成了一种新颖的检测硝基还原酶的双光子荧光探针。该探针是以香豆素奎唑啉酮为荧光团，对硝基苯为硝基还原酶的识别基团，在410 nm光激发下,该探针本身没有荧光，加入硝基还原酶后，探针在505
Institute of Chemical Biology and Nanomedicine, Hunan University, Changsha, 410006,Institute of Chemical Biology and Nanomedicine, Hunan University, Changsha, 410006,Institute of Chemical Biology and Nanomedicine, Hunan University, Changsha, 410006
#化学#
2014-12-17
基于碘离子与二溴代烯烃的特异性脱溴反应，设计合成了对碘离子具有高选择性的反应型荧光探针。此探针在 pH =7.4 的生理环境下, 有 I-的存在时，在680 nm处的荧光逐渐增强，检测的线性响应范围
高等学校博士学科点专项科研基金（20123704110004）
国家自然科学基金（21275092）
山东师范大学化学化工与材料科学学院，济南 250014,山东水利职业学院资源与环境系，日照 276826,山东师范大学化学化工与材料科学学院，济南 250014
#化学#
He Huaizhen,Lei Lei, Li Jianli,Cheng Zhao,Shi Zhen
In this study, a new kind of calcium-selective fluorescent probe Fluo-3ClMM-OH was synthesized. The structure of reaction products were characterized by IR and 1H NMR spectra. The molecular weights were measured on MALDI-TOF MS. Methylation of the crude product provided an effective method for separation. The fluorescence emission wavelength maxima of Fluo-3ClMM-OH were also researched.
2008-12-30
Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University
Intracellular viscosity strongly influences transportation of mass and signal, interactions between the biomacromolecules and diffusion of reactive metabolites in live cells. Herein, we developed a naphthalimide-hemicyanine based fluorescent probe Cyt-NA for imaging the change of microviscosity in living cells. Cyt-NA exhibited about 260 folds fluorescence increasing at 610 nm along with the increasing viscosity from 1.0 cP to 1410 cP. These satisfying response properties make Cyt-NA possible to monitor viscosity changes under starvation and low temperature conditions, offering us more practical information about microenviromental viscosity in living cells.
2017-04-28
Research Fund for the Doctoral Program of Higher Education of China （RFDP)（No. 20121101110049）
Beijing Institute of Technology, chemistry and chemical engineering, Beijing 100081,Beijing Institute of Technology, chemistry and chemical engineering, Beijing 100081
Detection of copper ions (Cu2+) is of great significance in protecting the environment and human health. In the present work, we developed a new colorimetric and fluorescent probe for Cu2+ determination with high selectivity and rapid-response rate. Upon Cu2+ addition, the probe underwent an 86 nm blue-shift in absorption peak wavelength as well as a significant quenching in fluorescence emission. The fluorescence intensity at 575 nm showed a good linear relationship (R2 = 0.999) with the Cu2+ concentration in the range of 0 μM ~ 12 μM. Using the probe as an analytical tool, the detection of Cu2 + can be done within 5 s, whose process was not subject to interference from other metal ions and pH changes. Moreover, the probe can be used for Cu2+ assay in both real water sample and living cells.
2017-05-05
Doctoral Program of Higher Education of China （No. 20121101110049）
Beijing Institute of Technology, Chemistry and chemical engineering, Beijing 100081, Beijing Institute of Technology, Chemistry and chemical engineering, Beijing 100081
2015-05-13
， Cu2+， Ag+， Mg2+ 和Zn2+）存在的影响，可作为具有Cu2+ 识别功能的荧光探针。
武汉理工大学化学化工与生命科学学院，湖北 武汉 430070,武汉理工大学化学化工与生命科学学院，湖北 武汉 430070
#化学#
2019-03-27
并合成了BODIPY类Galectin-3荧光探针。初步表征结果表明，所合成的探针与Galectin-3孵育后，其荧光偏振值有较大变化，具有用于Galectin-3抑制剂研究的潜力。
School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331,School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331,School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331
#化学#
2017-05-31
本工作设计了一种新型的双光子检测半胱氨酸（Cys）的荧光探针P2。该探针是通过将Cys响应基团丙烯酰基引入到双光子荧光团6-羟基-2-乙酰萘上设计而成的。在375 nm光激发下，该探针本身的荧光十分
湖南大学化学化工学院，长沙市 410082,湖南大学化学化工学院，长沙市 410082,湖南大学化学化工学院，长沙市 410082
#化学#
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