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2003-2021 全部
为您找到包含“钙离子”的内容共173

郝婷婷,付于

2013-11-28

目的:探讨针刺对快速老化鼠线粒体离子浓度和活性氧簇的调节。方法:运用快速老化鼠SAMP8和正常老化鼠SAMR1作为实验对象,采用"三焦针法"(原"益气调血,扶本培元"针法),应用荧光指示剂及荧光

国家自然科学基金面上项目(编号)8117341

天津市高校科技发展项目(编号)2009022

高等学校博士学科点专项科研基金(编号)2010121012000

天津中医药大学研究生院,天津 300193,天津中医药大学第一附属医院,天津 300193

#中医学与中药学#

Ma Haile,He Ronghai ,Wang Heli ,Luo Lin ,Wang Zhenbin

Transmembrane behavior of Ca2+ in E. coli cells impacted by high-intensity pulsed magnetic field (PMF) was studied. The bacteria were subjected to a treatment of PMF (0-4.5 T, 0-30 pulse numbers). The study found that intracellular calcium concentration ([Ca2+]i) of E. coli was changed significantly with PMF treatment. When sample was treated by PMF (2.5 T, 20 pulse numbers) at 30oC, total number colonies of E. coli 8099 sample decreased 63% while the [Ca2+]i increased 2.2 times as much as the sample without treatment. Change of [Ca2+]i in E. coli cells at logarithm phase is more sensitive to PMF treatment than those at other growth phases. There is certain relativity between transmembrane behavior of Ca2+ in E. coli cells and “biological window effect” corresponding to PMF sterilization effects, so the biological window effect of high-intensity PMF sterilization was validated preliminarily by transmembrane behavior of E. coli cell Ca2+. Effect of pulse number on [Ca2+]i is more placid than that of power. The increase of [Ca2+]i is an important reason of E. coli cells death in PMF sterilization.

2009-02-16

国家863计划重点项目(2007AA100405

Jiangsu University,Jiangsu University,Jiangsu University,Jiangsu University,Jiangsu University

#Food Science and Technology#

He Huaizhen,Lei Lei, Li Jianli,Cheng Zhao,Shi Zhen

In this study, a new kind of calcium-selective fluorescent probe Fluo-3ClMM-OH was synthesized. The structure of reaction products were characterized by IR and 1H NMR spectra. The molecular weights were measured on MALDI-TOF MS. Methylation of the crude product provided an effective method for separation. The fluorescence emission wavelength maxima of Fluo-3ClMM-OH were also researched.

2008-12-30

Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University

#Chemistry#

张静,曾申明

2013-02-17

本实验研究操作液中的离子浓度对猪ICSI胚胎表达EGFP效率的影响。冻融的精子和0.05μg/ml的pEGFP-N1DNA在4OC孵育1小时,然后分别在离子浓度为0,2,4,6,8mmol/L的

高等学校博士学科点专项科研基金资助课题(20090008110002

中国农业大学动物科技学院,北京,100193,中国农业大学动物科技学院,北京,100193

#畜牧科学、动物医学#

0评论(0 分享(0)

唐大伟,曾申明

2013-02-25

卵母细胞碎裂是卵母细胞在老化过程中的自发现象。研究表明离子作为细胞中的重要信使,不仅参与了卵母细胞的发育过程,也可能参与了卵母细胞的老化过程。因此,本实验通过检测在老化过程中猪卵母细胞的内质网形态

高等学校博士学科点专项科研基金资助课题(20090008110002

中国农业大学动物科技学院,北京 100193,中国农业大学动物科技学院,北京 100193

#畜牧科学、动物医学#

谢宁,何秀丽,周妍妍,姚辛敏

2009-01-21

组6组,分别加入正常培养液,正常脑脊液以及含药脑脊液,孵育后,加入离子荧光剂flou-3/AM负载后于激光共聚焦显微镜下观察离子在细胞内的含量。结果:含有脑脊液各组加入Aβ25-35后虽然荧光

国家自然科学基金(30472128

教育部博士点基金(20050228005

黑龙江中医药大学基础诊断教研室,黑龙江中医药大学基础诊断教研室,黑龙江中医药大学基础诊断教研室,黑龙江中医药大学基础诊断教研室

#中医学与中药学#