2013-11-28
目的：探讨针刺对快速老化鼠线粒体钙离子浓度和活性氧簇的调节。方法：运用快速老化鼠SAMP8和正常老化鼠SAMR1作为实验对象，采用"三焦针法"（原"益气调血，扶本培元"针法），应用荧光指示剂及荧光
国家自然科学基金面上项目（编号)8117341）
天津市高校科技发展项目（编号)2009022）
高等学校博士学科点专项科研基金（编号)2010121012000）
天津中医药大学研究生院，天津 300193,天津中医药大学第一附属医院，天津 300193
#中医学与中药学#
2008-01-23
混凝土是一种重要的建筑材料。实验和研究发现混凝土的含钙水化物，特别是氢氧化钙Ca(OH)2和水化硅酸钙C-S-H，在侵蚀性溶液作用下，其中的钙离子流失是混凝土结构力学性能劣化的重要原因。用加权余量法
河海大学
#土木建筑工程#
2009-01-16
以白玉兰花粉为材料，采用超纯水静置30min和花粉培养液水合30min两种预处理方式及常温（25℃）和低温（4℃）两种装载方法，将钙离子荧光探针Fluo-3, AM导入白玉兰花粉细胞。利用流式细胞仪
Ma Haile,He Ronghai ,Wang Heli ,Luo Lin ,Wang Zhenbin
Transmembrane behavior of Ca2+ in E. coli cells impacted by high-intensity pulsed magnetic field (PMF) was studied. The bacteria were subjected to a treatment of PMF (0-4.5 T, 0-30 pulse numbers). The study found that intracellular calcium concentration ([Ca2+]i) of E. coli was changed significantly with PMF treatment. When sample was treated by PMF (2.5 T, 20 pulse numbers) at 30oC, total number colonies of E. coli 8099 sample decreased 63% while the [Ca2+]i increased 2.2 times as much as the sample without treatment. Change of [Ca2+]i in E. coli cells at logarithm phase is more sensitive to PMF treatment than those at other growth phases. There is certain relativity between transmembrane behavior of Ca2+ in E. coli cells and “biological window effect” corresponding to PMF sterilization effects, so the biological window effect of high-intensity PMF sterilization was validated preliminarily by transmembrane behavior of E. coli cell Ca2+. Effect of pulse number on [Ca2+]i is more placid than that of power. The increase of [Ca2+]i is an important reason of E. coli cells death in PMF sterilization.
2009-02-16
国家863计划重点项目（2007AA100405）
Jiangsu University,Jiangsu University,Jiangsu University,Jiangsu University,Jiangsu University
He Huaizhen,Lei Lei, Li Jianli,Cheng Zhao,Shi Zhen
In this study, a new kind of calcium-selective fluorescent probe Fluo-3ClMM-OH was synthesized. The structure of reaction products were characterized by IR and 1H NMR spectra. The molecular weights were measured on MALDI-TOF MS. Methylation of the crude product provided an effective method for separation. The fluorescence emission wavelength maxima of Fluo-3ClMM-OH were also researched.
2008-12-30
Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University,Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University
2013-02-17
本实验研究操作液中的钙离子浓度对猪ICSI胚胎表达EGFP效率的影响。冻融的精子和0.05μg/ml的pEGFP-N1DNA在4OC孵育1小时，然后分别在钙离子浓度为0，2，4，6，8mmol/L的
2008-04-09
研究玉足海参糖胺聚糖（GAG）对血液中钙离子的影响。 方法 大白兔耳缘静脉取血进行体外复钙凝血实验，考察GAG对复钙凝血时间的影响。考查GAG浸泡24 h后的毛细管对小鼠眼眶后静脉丛取血过程中血液
2013-02-25
卵母细胞碎裂是卵母细胞在老化过程中的自发现象。研究表明钙离子作为细胞中的重要信使，不仅参与了卵母细胞的发育过程，也可能参与了卵母细胞的老化过程。因此，本实验通过检测在老化过程中猪卵母细胞的内质网形态
2009-01-21
组6组，分别加入正常培养液，正常脑脊液以及含药脑脊液，孵育后，加入钙离子荧光剂flou-3/AM负载后于激光共聚焦显微镜下观察钙离子在细胞内的含量。结果：含有脑脊液各组加入Aβ25-35后虽然荧光
国家自然科学基金（30472128）
教育部博士点基金（20050228005）
黑龙江中医药大学基础诊断教研室,黑龙江中医药大学基础诊断教研室,黑龙江中医药大学基础诊断教研室,黑龙江中医药大学基础诊断教研室
#中医学与中药学#
2009-02-09
“电磁污染”越来越引起科学界的关注，在本论文中我们从胞浆内钙离子浓度出发，扩展了Vm-[Ca2+]cyt 模型去解释在交变电场作用下，胞浆膜电位和胞浆内钙离子浓度的变化机制，外部的交变电场会对细胞膜
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