Cell wall is an important subcellular component of dinoflgaellate cells in regard to various aspects of cell surface associated ecophysiology but the full range of cell wall proteins (CWPs) and their functions remain to be elucidated. This study identified and characterized CWPs of a toxic dinoflagellate, Alexandrium catenella using a combination of 2-D fluorescence difference gel electrophoresis (DIGE) and MALDI TOF-TOF mass spectrometry approaches. Using sequential extraction and temperature shock methods, sequentially extracted CWPs and protoplast proteins were separated from A. catenella, respectively. Comparison between sequentially extracted CWPs labeled with Cy3 and protoplast proteins labeled with Cy5, 120 CWPs were confidentially identified in the 2-D DIGE gel. These proteins gave positive identification of protein orthologues in the protein database by de novo sequence analysis and homology-based search. Majority of the prominent CWPs identified were hypothetical or putative proteins with unknown function or no annotation, while cell wall modification enzymes, cell wall structural proteins, transporter/binding proteins, signaling and defense proteins were tentatively identified in agreement with the expected role of the extracellular matrix in cell physiology. This work represents the first attempt to investigate CWPs of dinoflagellates which provides a potential tool for future comprehensive characterization of CWPs and elucidation of physiological functions of CWPs in dinoflagellates.
Specialized Research Fund for the Doctoral Program of Higher Education （20070384014)）
State Key Laboratory of Marine Environmental Science/Environmental Science Research Centre, Xiamen
本文收录在Evid Based Complement Alternat Med. 2011;2011:984080. Epub 2011 Sep 5.
A novel magnetically separable core/shell type nanocomposite was synthesized in a facile way and characterized by XRD, UV-Vis, FT-IR, TEM, Nitrogen physisorption and Magnetite susceptibility measurements. In the synthesis procedure, the mesoporous silica shell was coating on the surface of magnetic core directly via a nano-assembling method. After calcination at high temperature (550 oC) for template removal, the spinel phase of maghemite core was retained without any transformation. The resulting material possessed a regularly hexagonal mesoporous structure with a high specific surface area (908.70 m2/g), which were favorable to further functional modification. Once incorporated with iron species by in situ synthesis, the nanocomposite could serve as a Fenton catalyst and was effective in phenol degradation at the given conditions (40 oC, pH=4). At the end of the reaction, it was easily collected by an external magnetic field and remained efficient in reuse.
Specialized research fund for the doctoral program of higher education（SRFDP）
School of Environmental Science and Engineering, Shanghai Jiao Tong University,School of
The effects of three surfactants (Tween 80, saponin and monorhamnolipid) on the hydrolysis of NaOH-pretreated rice straw by low dosage of cellulase were studied. The results indicated that with a relatively low cellulase dosage (4 FPU g-1 substrate), all surfactants were able to enhance the enzymatic hydrolysis, while the biosurfactant monorhamnolipid was demonstrated to be more active than Tween 80 and saponin. Monorhamnolipid at 0.006% increased reducing sugar yield by 23.15%, and the maximal sugar yield was obtained within half of the time compare to the control required. The cellulase losed 67% of its activity in the control, while the activity lost ratios were 31%~43% in the presence of the surfactants. The surfactants addition did not affect the pH of the hydrolysis system. This study provides an opportunity of decreasing enzyme dosage in hydrolysis of agricultural solid waste rich in cellulose.
Environmental Science and Engineering,Hunan University,Key Laboratory of Environmental Biology and Pollution
In this paper, ROM model, a kinetics model on organic substrate removal, was established on the basis of material balance in submerged membrane bioreactor (SMBR). According to the model, the organic substrate removal rate is determined by not only the organic substrate concentrations in influent and effluent, hydraulic retention time (HRT), but also the organic substrate concentration removed by membrane module and sludge retention time (SRT). Meanwhile, SRT and the maximum value of mixed liquor volatile suspended solids (MLVSS) without discharging sludge can be determined as well as the concentration of MLVSS can be predicted in reactor. Brewery wastewater was chosen and examined to verify the model and the results showed that the experimental data were coincident with the calculating data.
Beijing Forestry University Technology Innovation Program （YX2011-12）
Program for New Century Excellent Talents in University（NCET-08-0732）
Fund for Doctor Stations（20090014120018）
Ministry of Education（0）
College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083
The oxidation processes of Cr(III) to Cr(VI) are not well understood, although the reduction of Cr(VI) has been extensively investigated. Here the effects of the small organic acid, formic acid, on the iron-catalyzed photo-oxidation of Cr(III) were examined. The concentrations of Cr(VI), Fe(II) and H2O2 were monitored to elucidate the reaction mechanisms. In the absence of formate, Cr(III) can be readily oxidized to Cr(VI) by photogenerated oOH from photolysis of ferric hydroxyl complex. The presence of formate diminished the production of Cr(VI) due to its strong radical scavenging capacity for oOH and potential re-reduction of Cr(VI) by its secondary reducing CO2o- radical. However, the yield of CO2o- may be very low because of the much lower quantum yield of oOH in UV/Fe(III) systems. The present study has important implication for understanding the redox chemistry of chromium species in carbon-rich aquatic environment.
Research Fund for the Doctoral Program of Higher Education of China （No. 20100075120010) and the Fundamental Research Funds for the Central Universities）
College of Environmental Science and Engineering, Donghua University, Shanghai 201620,College of
A realistic case study was conducted in Tangail Municipal Area (TMA), the most oldest and fast growing semi-urban town of Bangladesh. The study emphasized to investigate the environmental conditions and problems have been faced by inhabitants. The study was performed through both experimental and perceptional basis. The study revealed that about 86.7% household have no access to dustbin, 68.7% have no outlet to drain out waste water, and only 15.3% get supply water. The study also finds that, about 3000-3500 kg/day of hospital waste are generated from more than 40 clinics and hospitals. Noise intensity is higher than prescribed levels and its frequency from 57.0 to 112.3 dB (A) at different areas in TMA. Due to the disposal of waste water, effluent from factories, hospital and solid waste in the river inside the town, the water is also polluted. For unhealthy environmental condition of town dwellers, they were suffering several frequent diseases.
Department of Environmental Science and Resource Management, Mawlana Bhashani Science and
This paper reports on a seasonal pattern comparison of microbial enzymatic activities and biomass responses based on a conventional biofilter (BF, without earthworm) and a vermi?lter (VF, with earthworm, Eisenia fetida) for sewage sludge treatment fed continuously. The VSS reduction, viable cell number and enzyme activities were assayed to probe what made the VF operated stably. The results indicated that the earthworms' activities can further the volatile suspended solids (VSS) reduction with a 27.17% more than BF. Though the VF has a lower level in the viable cell number, the earthworms strongly improved the enzyme activities, such as INT- dehydrogenase, protease, β-glucosidase and amylase, which can explain the excellent performance of VSS reduction, compared with the BF. The correlation analysis documented that the VSS reduction was positively correlated with microbial enzyme activities. More importantly, the earthworm enabled the VF to avoid the detrimental influence of temperature, which guaranteed a stable performance during seasonal variations.
the PhD Programs Foundation of Ministry of Education of China （No. 20110072120029）
College of Environmental Science and Engineering, Tongji University, 200092,College of
This paper studies remote argon plasma sterilization to medical Poly(tetrafluoroethylene)(PTFE) film surface contaminated with E.coli and characterized surface structure, performances of PTFE film by the water contact angle, mass loss and platelet adhesion measurements as well as X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Results show that both remote and direct argon plasmas can sterilize effectively (GE ≥3.769) at the conditions of RF power 100W, exposure time 120s and argon flux 20 cm3/min. But the remote argon plasma sterilization contributes more effectively to the defluorination(F/C = 2.2425) from the PTFE film surface than that of the direct one contributes(F/C = 2.4853) and introduces more oxygen-containing groups (e. g. C=O) into it, makes this surface higher hydrophilicity and better antithrombogenicity while degradation and damages least, biocompatibility most excellent. Both the optimal inactivation and surface performances of PTFE film for use in medicine can be obtained simultaneously by remote argon plasma sterilization. The essential reason is that remote argon plasma sterilization can enhance interaction reactions with argon radicals relative to those with electron and argon ions.
Department of Environmental Science and Engineering and Engineering,Xi’an Jiaotong University
NaNH2 and NaBH4 are structured as a composite hydrogen storage material, NaNH2-NaBH4. To improve the thermal decomposition kinetics of NaNH2-NaBH4, which is crucial to hydrogen releasing performance, Ni-Co-B is selected as a catalyst. To get the catalyst-promoted light-weight hydrogen storage materials, the composite hydrogen storage system was synthesized via a ball milling method with NaNH2, NaBH4 and Ni-Co-B. X-ray diffraction patterns show that the crystal structure of the NaNH2-NaBH4 is well kept after adding catalysts. In addition, Na3BN2 and metal Na are detected as a by-product in the Ni-Co-B promoted NaNH2-NaBH4 (2/1) through the thermal decomposition process. In addition, TG-DTA analysis indicates that the main decomposition stages of the metal boride promoted NaNH2-NaBH4 materials are split into three stages. Furthermore, the catalytic effects on the thermal decomposition kinetics are evaluated. The activation energy of the Ni-Co-B promoted NaNH2-NaBH4 below 250oC is 99KJ/mol, which are only 62.0% of that of the pristine NaNH2-NaBH4 (2/1), and implying better kinetics at low temperature decomposition range.
Research Fund for the Doctoral Program of Higher Education of China （20121101110042）
National Natural Science Foundation（20806010）
Program for New Century Excellent Talents in University（NCET-12-0047）
Beijing Key Laboratory of Environmental Science and Engineering, School of Chemical Engineering and
(Although the corona electrostatic separation is successfully used in recycling waste printed circuit boards in industrial applications in China, there are problems which can't be resolved completely, such as nonmetal particle aggregation and high-voltage breakdown. Both of these impact the process of separation and are not easy to identify during industrial application. This paper provides a systematic study on a real-time monitoring system. A real-time weight monitoring system was established to continuously monitor the separation process. The monitoring system comprised of two parts: hardware and software. A Virtual Instrument (VI) program written by LabVIEW was used to sample and analyze the incremental accumulation of middling. It included:1) A Real-time monitoring system for the output of corona electrostatic separation (CES); 2) Real-time display of the weight accumulation of middling, the automatic determination to ensure middling stay within the limits ; 3) History date saving to file, which can be used by other software; 4) Statistic analysis and control for the incremental accumulation of middling. Three kinds of work conditions were used to verify the monitoring system. It was found that the system achieved continuous monitoring during the separation process and analyzed the received data. The system also gave comprehensible feedback for the accidents (material blockage in the feed inlet and high-voltage breakdown). The alarm signals not only helped operators to solve the problems in time, but provided input signals for improving the intelligence of the automatic line. From the alarm system, the monitoring system also saved human cost and helped the new technology to replace primordial methods as quickly as possible.
Research Fund for the Doctoral Program of Higher Education（20090073120041）
National Natural Science Foundation of China （51008192）
School of Environmental Science and Engineering, Shanghai Jiao Tong university, ShangHai 200240