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2003-2020 全部
为您找到包含“RNA结合蛋白”的内容共16

WANG Xubo,JIANG Jiajun,ZHANG Quanqi

DAZL is an RNA-binding protein of DAZ family, playing key roles in reproduction of organisms. Here in this study, we clone and identify the dazl gene in a kind of flatfish, Japanese flounder (Paralichthys olivaceus). Two different splicing variants of dazl mRNAs are found, and they differ from each other by a 51-bp insertion/deletion. We characterize the two mRNA variants by qPCR and in situ hybridization and prove their germ cell specific expression patterns. Results of western blot and immunohistochemistry show that the two isoforms of their translational production also express specifically in germ cells of both sexes. The similar expression patterns of the two variants may imply their complementary functions in germ cell development. But there are also differences in their functions. By RNA-binding protein immunoprecipitation, we find Podazl-I and Podazl-II may be the target of both PODAZL isoforms. More sequencing results will offer more detailed and accurate information on their functions. Studies on the two dazl variants in Japanese flounder are rare in teleosts. Our results may facilitate further studies on reproduction related genes and germ cell development.

2016-05-23

Doctoral Fund of Ministry of Education of China (No.20120132110010

College of Marine Life Science, Ocean University of China,College of Marine Life Science, Ocean University of China,College of Marine Life Science, Ocean University of China

#Biology#

0评论(0 分享(0)

刘莉,陈剑清,吴杰,吴祥甫,张耀洲

2009-02-12

RNA监视,也称无意义密码子介导的mRNA的降解(nonsense mediated mRNA decay, NMD) 。Y14是一类在真核RNA监视中起主要作用的RNA结合蛋白。我们从家蚕

国家973计划课题(No.2005CB121006

国家863计划课题(No.2007AA021703

国家863计划课题(No.2007AA100504

浙江理工大学生命科学院,浙江理工大学生命科学院,浙江理工大学生命科学院,浙江理工大学生命科学院,浙江理工大学生命科学院

#生物学#

0评论(0 分享(0)

Mei Zhuzhong,Li Tao,Wang Ni,Chen Xinyu,Ou Xiaoli,Jiang Yong

IκBα, encoded by NFKBIA, is the prototype inhibitor of transcription factor NF-κB. Notably, the transcription of NFKBIA is directly regulated by NF-κB. In contrast, the contribution of post-transcriptional regulation in the expression of NFKBIA remains obscure. To elucidate the post-transcriptional regulation of NFKBIA, we first aligned the 3?-UTR from several different species (human, mouse, rat, dog and cow). The alignment results revealed that the 3?-UTR sequences were highly conserved among these analyzed species. Dual luciferase assay analysis showed that 3?-UTR of mouse NFKBIA downregulated the expression of associated luciferase gene. Heat shock treatment stabilized NFKBIA mRNA and promote the expression of luciferase gene associated with mouse NFKBIA 3?-UTR. Biotin-labeled RNA pulldown assay followed mass spectrometry analysis identified specific binding proteins to the 3?-UTR of mouse NFKBIA mRNA. Half of them were identified as ribosomal proteins of 40S and 60S ribosome subunits.

2014-01-15

国家自然科学基金 (81070955

教育部新教师基金项目(20104433120009

Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Southern Medical University,Guangzhou 510515,China,Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Southern Medical University,Guangzhou 510515,China,Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Southern Medical University,Guangzhou 510515,China,Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Southern Medical University,Guangzhou 510515,China,Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Southern Medical University,Guangzhou 510515,China,Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Southern Me

#Basic Medicine#

0评论(0 分享(0)

苏位君,李帅

2017-05-16

miRNA是一类具有重要调控功能的小分子非编码RNA,广泛参与发育、细胞增殖、凋亡、周期等多种重要生物学过程的调节。Dicer是miRNA加工成熟过程中重要的节点蛋白。双链RNA结合蛋白TRBP

高等学校博士点专项科研基金——新教师类(20131202120002

天津市应用基础与前沿技术研究计划——青年项目(15JCQNJC11700

南开大学医学院,天津医科大学肿瘤医院乳腺病理研究室,国家肿瘤临床医学研究中心,天津市

#生物学#

Zhu liyuan,Ruan Xiangbin,Wu Fan,Qi Yingjiao,Zhou junjie,Liu Wei,Li liang,Zhang jing,Yin Bin,Jiang Tao,Yuan Jiangang,Qiang Boqin,Han Wei,Peng Xiaozhong

Glioma is the most common primary malignancy in the brain, which has the high recurrence and lethality rate, and it's imminent to explore the molecular mechanism of this incurable disease. Poly-pyrimidine tract binding protein (PTB, also known as PTBP1) is a kind of RNA-binding protein with various molecular functions and plays an indispensable role in glioma. Since research about the regulation of PTB is limited in microRNAs and transcription factors, here we want to explore the new lncRNA regulators of PTB in order to consummate the principle of controlling the PTB expression and further reveal the molecular mechanism of accommodating glioma tumorigenesis. We identify a novel natural antisense transcript of PTB named PTB-AS and find that the expression of PTB-AS in glioma is significantly directly correlated with PTB-mRNA. Naturally, knocking down the abundance of PTB-AS in glioma can remarkably reduce the expression of PTB mRNA and protein. Then we validate the important function of PTB-AS in glioma for the first time and discover the original fact that PTB-AS influence the stability of PTB-mRNA by directly binding to PTB-3'UTR, what's more, it's the latest finding to demonstrate that miR-9 can negatively regulate PTB in human cancer and in practice PTB-AS could elevate the expression of PTB by masking the binding site of miR-9 in PTB-3'UTR so as to maintain the high expression level of PTB, miR-9 and itself in glioma.

2016-03-18

高等学校博士学科点专项科研基金新教师类资助课题(20131106120021

State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China,State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China,State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China,State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China,State Key Laboratory

#Basic Medicine#

0评论(0 分享(0)

贾军祥,张风群,黄云仙,章小斌,吴怡,丁明,赵辅昆

2012-01-18

Vigilin 是一个RNA结合蛋白,由 14 个连续的RNA结合结构域--- KH 结构域组成。Vigilin 可以通过与 mRNA 3’UTR 结合调控 mRNA 的翻译。本文通过 EMSA

浙江理工大学生命科学学院,杭州 310018,浙江理工大学生命科学学院,杭州 310018,浙江理工大学生命科学学院,杭州 310018,浙江理工大学生命科学学院,杭州 310018,浙江理工大学生命科学学院,杭州 310018,浙江理工大学生命科学学院,杭州 310018,浙江理工大学生命科学学院,杭州 310018

#生物学#

0评论(0 分享(0)

周英利,袁梦求,胡欣,吴功伟,高平

2017-05-31

;结果:circRNA与RNA结合蛋白Lin28存在相互结合;成功构建circRNA过表达载体;circRNA在肝癌细胞系中有显著的过表达效果;circRNA过表达的肝癌细胞系中Lin28蛋白表达下降

高等学校博士学科点专项科研基金(20133402110014;20133402110020

中国科学技术大学生命科学学院,合肥 230027,中国科学技术大学生命科学学院,合肥 230027,中国科学技术大学生命科学学院,合肥 230027,中国科学技术大学生命科学学院,合肥 230027,中国科学技术大学生命科学学院,合肥 230027

#生物学#

2评论(0 分享(0)

WANG Zhonghui,LI Shujing,Qi Yao,LIU Xiaoyan,ZHAO Jingjing,HAN Yu,CHEN Xianhua,XU Ping

The candidate plasticity related gene 15 (cpg15) plays important roles in neural development and plasticity. In the present study, we studied the role of the cpg15 3'-untranslated region (UTR) in regulating the expression of the gene. The results showed that the presence of the 3'-UTR significantly decreases, while the absence of the 3'-UTR increases the cpg15 expression, indicating that the 3'-UTR may be critical for regulation of cpg15 expression. In addition, HuD, a neural-specific RNA binding protein, increased the cpg15 expression, which depends on the presence of the 3'-UTR. The results suggest that HuD regulates the cpg15 expression via the 3'-UTR-mediated mechanism.

2011-01-19

National Natural Science Foundation of China (No. 30971464

State Key Laboratory of Medical Neurobiology,Fudan University,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032, China,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032, China,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032, China,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032, China,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032, China,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032, China,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032, China

#Biology#

0评论(0 分享(0)

王慧娟,兰洋,刘涛,李灵

2010-12-22

PSF(polypyrimidine tract binding protein-associated splicing factor, PSF)蛋白是一种多功能的DNA/RNA结合蛋白,它能够抑制

国家自然科学基金(31000579

海南省自然科学基金 (310045

高等学校博士学科点专项科研基金(20070610170

四川大学生命科学学院功能基因组实验室,成都 610064,四川大学生命科学学院功能基因组实验室,成都 610064,四川大学生命科学学院功能基因组实验室,成都 610064,四川大学生命科学学院功能基因组实验室,成都 610064;海南医学院海南省热带病重点实验室,海口 571101

#生物学#

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