The shortage of available genomic and transcriptomic data hampers the molecular study on the locust central nervous system (CNS). Here, locust CNS RNA was sequenced by deep sequencing; 41,179 unigenes were obtained with an average length of 570 bp, and 5,519 unigenes were longer than 1,000 bp. A total of 15,895 unigenes were identified, including 644 nervous system relevant unigenes. Among the 25,284 unknown unigenes, 9,482 were found to be specific to CNS by filtering out the previous ESTs acquired from locust organs without CNS. The locust CNS showed the most matches (18%) with Tribolium castaneum sequences. Comprehensive assessment reveals that the database generated in this study is broadly representative of the CNS of adult locust, providing comprehensive gene information at the transcriptional level that could facilitate our research of the locust CNS, including various physiological aspects and pesticide target finding.
2011-04-19
Research Fund for the Doctoral Program of Higher Education of China （No. 20090191110031）
Natural Science Foundation of China （No. 30971913）
Chongqing University Postgraduates’Innovative Team Building Project（Team number: 200909B1009）
Genetic Engineering Research Center, School of Bioengineering, Chongqing University,Genetic Engineering Research Center, School of Bioengineering, Chongqing University
#Biology#
2016-11-23
。本研究立足于实验室所建立的小报春高通量转录组测序数据，根据从中获取的Unigene设计G10H基因引物，克隆出基因全长共1592bp，开放阅读框1500bp，编码499个氨基酸。将该氨基酸序列与其
高等学校博士学科点专项科研基金（20135103120014）
四川农业大学风景园林学院，成都 611130,四川农业大学风景园林学院，成都 611130,四川农业大学风景园林学院，成都 611130,四川农业大学风景园林学院，成都 611130
#林学#
2007-06-25
，对家鸡dbEST以及nr核酸数据库进行BLAST检索，发现该序列为一未知功能的EST序列，属于UniGene数据库中Gga.8499基因簇。下载该基因簇所有序列，利用CAP3序列拼接程序获得10个基序
2014-05-30
利用RT-PCR技术，将牡丹花瓣转录组数据库中7个Unigene序列进行拼接，得到3个bHLH基因，分别命名为PsbHLH1、PsbHLH2和PsbHLH3。设计特异性引物对3个基因最大阅读框
高等学校博士学科点专项科研基金（20130014110014）
北京林业大学园林学院，国家花卉工程技术研究中心，北京100083,北京林业大学园林学院，国家花卉工程技术研究中心，北京100083
#林学#
金邦泽,于威,盛清,吕正兵,陈健,聂作明,王丹,刘立丽,沈红丹,舒建洪,陈剑清,吴祥甫,张耀洲
2009-02-12
在自建的家蚕蛹 cDNA文库中发现了一条新基因序列，GenBank登录号为DY231426。Unigene分析预测该基因只在蛹期表达，因此将其命名为pupa-sepecific
国家自然科学基金（No. 2005CB121006）
国家自然科学基金（No. 2007AA021703）
国家自然科学基金（No. 2007AA100504）
浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,中科院上海生命科学学院生物化学与细胞生物学研究所,浙江理工大学生物化学研究所
#生物学#
2012-11-15
以"荸荠"杨梅四个不同成熟度的果实为材料，构建了四个cDNA文库，并分别随机挑选500个克隆进行测序。对这2000条测序得到的表达序列标签（EST）序列进行拼接共获得了395个Unigene，基于
国家公益性行业科研专项（201203089）
浙江省教育厅科研项目（Y201122362）
高等学校博士点基金项目（20090101110099）
浙江省科学技术厅国际科技重点合作项目（2009C14023）
浙江大学果实品质生物学实验室，农业部园艺作物生长发育与品质生物学实验室，杭州 310058,浙江大学果实品质生物学实验室，农业部园艺作物生长发育与品质生物学实验室，杭州 310058,浙江大学果实品质生物学实验室，农业部园艺作物生长发育与品质生物学实验室，杭州 310058,浙江大学果实品质生物学实验室，农业部园艺作物生长发育与品质生物学实验室，杭州 310058,浙江大学果实品质生物学实验室，农业部园艺作物生长发育与品质生物学实验室，杭州 310058,浙江大学果实品质生物学实验室，农业部园艺作物生长发育与品质生物学实验室，杭州 310058
#农学#
LI Peng,JIN Fangcao,ZHOU Kaiya
revealed 76112 unigene. In total 28,129 of the putative protein unigenes showed significant similarity to
2013-07-25
Foundations: National Natural Science Foundation of China （31000954）
Research Fund for the Doctoral Program of Higher Education of China （No. 20103207120007）
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023,Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China,Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China
#Biology#
ZHUANG Jing,HUANG Juxiang,WANG Lin
Ontology, KEGG, BioCarta, GenMapp, Intact, UniGene, OMIM, etc. By compared the different activated
2018-08-28
National Natural Science Youth Fundation of China （No. 81501372）
School of Electronic Engineering, Beijing University of Posts and Telecommunications, 100876;School of Electronic Engineering, Beijing University of Posts and Telecommunications, 100876;School of Electronic Engineering, Beijing University of Posts and Telecommunications, 100876,,
#Biology#
2012-09-05
代自交系'四九菜心'为母本，利用国际公用简单重复序列(Simple sequence repeats, SSR)标记和本实验室设计开发的单基因微卫星(Unigene microsatelite
教育部高等学校博士学科点专项科研基金（20092013110006）
沈阳农业大学园艺学院，沈阳 110866,沈阳农业大学园艺学院，沈阳 110866,沈阳农业大学园艺学院，沈阳 110866
#农学#
本文收录在中国科技论文在线精品论文，2013，6（19）：1814-1819.
selected, and oligoprobes were then designed with the UniGene Database and the Human Reference Sequence
2017-05-16
Research Fund for the Doctoral Program of Higher Education of China （no. 20130001110078）
Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, 100044,Department of Obstetrics and Gynecology, Peking University Peopl
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