2018-03-08
目的：探讨香烟烟雾致人支气管上皮细胞（BEAS-2B）恶性转化过程中组蛋白乙酰化改变及其对FOXO3基因表达的影响。方法：以BEAS-2B细胞作为研究对象，建立香烟暴露致恶性转化的体外模型；利用流式细胞术检测细胞凋亡，检测caspase3/7活性改变；高通量ChIP-Seq检测恶性转化细胞中组蛋白H3K9乙酰化改变，Realtime-PCR和Western blotting检测FOXO3及其下游基因的mRNA和蛋白表达。结果：随染烟程度增加，染毒细胞caspase3/7活性与凋亡率逐渐降低；基因启动子区组蛋白H3K9乙酰化分别有155个升高和892个降低，其中组蛋白低乙酰化的抗凋亡基因FOXO3表达下降，组蛋白去乙酰化酶抑制剂（TSA）处理后表达回复；FOXO3下游凋亡相关基因表达降低。结论：组蛋白H3K9低乙酰化导致FOXO3基因表达降低，在香烟烟雾致BEAS-2B细胞恶性转化的凋亡逃逸过程起着重要作用。
国家自然科学基金（81573178）
国家自然科学基金（81172707）
国家自然科学基金（81573178）
国家自然科学基金（81172707）
国家自然科学基金（81573178，81172707）
国家自然科学基金（81573178）
国家自然科学基金（81172707）
苏州大学医学部公共卫生学院，苏州 215123,苏州大学医学部公共卫生学院，苏州 215123,苏州大学医学部公共卫生学院，苏州 215123,苏州大学医学部公共卫生学院，苏州 215123
#预防医学与卫生学#
2009-11-24
钙整合素结合蛋白一般含有4个EF-hand结构域，可与包括血小板αⅡbβ3 integrin 等许多效应蛋白结合并能调节他们的功能。我们从本实验室构建的家蚕蛹cDNA文库中获得一条cDNA序列，通过同源序列比对后发现其为家蚕钙整合素结合蛋白 (Bombyx mori calcium and integrin binding protein，BmCIB)基因, 全长为939 bp，ORF为558 bp，编码165个氨基酸。我们构建了重组表达载体pET28a- BmCIB，在E. coli BL21(DE3)中诱导表达了含有His标签的融合蛋白BmCIB。通过包涵体纯化与亲和层析法进一步纯化融合蛋白并免疫雄性新西兰兔，制备并获得了多克隆抗体。通过实时定量PCR和Western blot分析，我们发现BmCIB 的mRNA和表达的蛋白在五龄家蚕各组织及发育时期广泛分布。其mRNA转录水平以生殖腺和蛹期中最高，蛋白表达在各发育时期均可检测到，各组织中以生殖腺中最多。通过免疫荧光法分析BmCIB蛋白在家蚕Bm5细胞的亚细胞定位，发现其定位于细胞质中。以上工作为进一步研究BmCIB功能奠定了基础。
国家自然科学基金（2007AA021703；2007AA120402）
国家自然科学基金（2005CB121006）
浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所,浙江理工大学生物化学研究所
#生物学#
We demonstrate theoretically that it is possible to stop quantum light in vertically coupled InGaAs/GaAs quantum dots systems simply by applying an external voltage. This stopping is based on the form-stable polaritons consisting of quantum light and indirect exciton associated with electromagnetically induced transparency using tunneling instead of pump laser. The indirect exciton dark-state polaritons in the two coupled quantum dots can then be reaccelerated and converted back into a photon pulse via a tunable voltage. The device system has possible applications in quantum information processing by using modern electro-optical technology.
2009-11-30
国家自然科学基金（10774101）
国家自然科学基金（10974133）
Department of Physics, Shanghai Jiao Tong University,Department of Physics, Shanghai Jiao Tong University
#Physics#
We theoretically propose a scheme to study coherent population oscillation (CPO) in a system of a quantum dot coupled to nanomechanical resonator. Due to nanomechanical vibrations the CPO in a single quantum dot demonstrates novel features. In analogy with CPO in quantum optics, we refer this effect as mechanically induced coherent population oscillation (MICPO). In this scheme, the vibration of the nanomechanical resonator make a contribution to additional auxiliary energy levels so that the transparency phenomenon could be realized in such system. The optical spectrum shows that the transparency based on MICPO can be controlled simply by the intensity of the optical field, the coupling strength and other relevant parameters. Furthermore, our technique provides a detection in a real experiment to measure the decay rate of nanomechanical resonator.
2009-12-01
国家自然科学基金（10774101）
国家自然科学基金（10974133）
Department of Physics, Shanghai Jiao Tong University.,Department of Physics, Shanghai Jiao Tong University.
#Physics#
2010-01-12
最初定义的埃达克岩是钠质火成岩，其全岩化学成分相当于英云闪长岩、奥长花岗岩和（富斜长石的）花岗闪长岩（TTG）；而富钾的“C型埃达克岩”全岩化学成分相当于（狭义）花岗岩、石英二长岩和（富碱性长石的）花岗闪长岩。目前的实验岩石学结果不支持玄武质源岩部分熔融形成SiO2含量中等（56 ~ 67 %）的“C型埃达克岩”的假设。但中等富钾程度的贫硅（或高Mg#值）玄武质源岩在相对高压低比例部分熔融条件下可以形成酸性（SiO2 > 70%）“C型埃达克岩”。
国家自然科学基金（0）
国家自然科学基金（0）
1) 中国地质大学地质过程与矿产资源国家重点实验室，北京100083；2) 中国地质大学（北京）岩石矿物教研室，北京100083,1) 中国地质大学地质过程与矿产资源国家重点实验室，北京100083；2) 中国地质大学（北京）岩石矿物教研室，北京100083
#地球科学#
2010-01-12
为了观察基底刚度对表皮细胞增殖、迁移的影响，将表皮细胞接种在三种不同刚度的硅胶基底上。通过细胞生长曲线、MTT法和细胞周期检测表皮细胞的增殖情况；用细胞划痕法进行表皮细胞迁移率的测定。结果发现表皮细胞的增殖速度在高刚度基底上更快，同时在高刚度基底上迁移率远远高于其在低刚度基底上的迁移率。推测可能是基底力学性质的变化引起整合素介导的力学信号发生改变，从而调控表皮细胞的增殖与迁移。
国家自然科学基金（30730093）
国家自然科学基金（30970721）
生物流变科学与技术教育部重点实验室，重庆大学生物工程学院,生物流变科学与技术教育部重点实验室，重庆大学生物工程学院,第三军医大学创伤、烧伤与复合伤国家重点实验室,生物流变科学与技术教育部重点实验室，重庆大学生物工程学院
#生物学#
Jia Yanyan ,Qin Jiachang ,Zhou Changfa
Because of its large species diversity, less characters for classification, smaller body and confused taxonomic systems as well as history, the Baetidae is a very complicated taxon in mayfly taxonomy and phylogeny reconstruction. In recent years, some species from Zijin Hill of Nanjing city (Jiangsu Province, Eastern China) are differentiated and identified upon adult-nymph association by rearing in lab. From prostheca of right mandible (as two spines) and hindwings (having 3 longitudinal veins), the species Baetis yixiani Gui and Lu, 1999 is transferred into the genus Alainites Waltz and McCafferty (1994) hereby. More reports and descriptions on Baetidae from Chinese mainland are preparing and will be published later. Hopefully, series papers on this issue can clarify some problems in mayfly taxonomy of China, or even facilitate and boost more researches as well.
2010-03-18
国家自然科学基金;国家自然科学基金（30300037;30570200）
The Key Laboratory of Jiangsu Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China,The Key Laboratory of Jiangsu Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China,The Key Laboratory of Jiangsu Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China
#Biology#
Zhao Zihua ,He Dahan ,Zhao Yingshu ,Hang Jia ,Shi Xiangfeng
Minimum Area of Suitable Habitat (MASH) and Minimun Viable Population (MVP) were two important aspects in Population Viability Analysis (PVA). MASH for a viable population can be estimated in several ways. The density-area method estimated MASH as the smallest area in which population can survival for a long time and variation was below 0.05. We used a variant of density-area method to study MASH for aphids, parasitoids, and hyperparasitoids in Northwest of China, where wheat was a main crop in agriculture. The variant was based on the premise that individuals within populations were likely to occur at usually high densities variation when confined to small areas and it estimated MASH as the smallest area beyond which density plateaus. For all 72 sites, aphids, parasitoids and hyperparasitoids occurred in all sites because wheat fields were highly homogeneous. We found that a consistant inverse density-area relationship was present over smaller areas, but different species had different function, especially in different trophic level. MASH of M. avenae, S. graminum, A. avenae, A. gifuensis, A. sp.1 and P. aphidis estimated from density-area relationship were 260, 240, 510, 490, 950, and 990m2 respectially. Results of inverse proportion function according to which MASH of M. avenae, S. graminum, A. avenae, A. gifuensis, A. sp.1 and P. aphidis were 310, 286, 543, 492, 952, and 1003 m2 respectially were similar to that of density-area relationship. We concluded that a negative density-area relationship may be an inevitable consequence of agricultural intensification and farmland fragmentation. We also concluded that different species may have different MASH requirement in agricultural landscape according to body size, migration, trophic level, and habitat quality, which could interpret the phenomenon that highest percent parasitism was always in 800-1000 m2 wheat fields which exceeded MASH of parasitoids and suppressed activity of hyperparasitoids . Finally, we concluded that the value of any MASH as a pest control tool was compromised in Conservation Biological Control (CBC).
2010-03-29
国家自然科学基金（30760045）
国家自然科学基金（30860164）
Institute of Grassland Sciences, Ningxia University,Institute of Grassland Sciences, Ningxia University,Institute of Grassland Sciences, Ningxia University,Institute of Grassland Sciences, Ningxia University,Institute of Grassland Sciences, Ningxia University
#Biology#
2010-04-22
针对码书模型因算法复杂度高而难以应用到运动目标实时检测中的问题，提出了一种改进的码书建模方法。首先优化了码书模型的参数和训练策略，然后提出一种信息块快速获取策略，利用该策略对获得的目标信息块和时间轴运动信息块进行融合决策，实现了在检测过程中实时分离背景和训练码书模型。实验结果表明：经优化的码书建模方法处理效率大幅度提高的同时，检测性能与原始码书模型一致，能对多变环境快速适应，有效解决了码书建模的实时运动目标检测问题。
2017-01-11
对于CHES2015上提出的新的轻量级分组密码算法Simeck，目前的分析结果主要集中在差分攻击，线性攻击等，而对于该算法在不可能差分攻击下的抵御情况，还没有足够的研究。因此，本文主要分析Simeck算法对不可能差分攻击的抵御情况。首先，对于Simeck算法特殊的非线性组件，提出了简单的由输入差分直接计算输出差分通式和概率的算法；然后根据这一算法，给出了多条不可能差分路径；并且讨论了运用多条路径对攻击复杂度的影响，从而选择最优的路径数减小攻击复杂度。本文对Simeck32/64, Simeck48/96, Simeck64/128给出了新的不可能差分攻击结果，相比原有结果，具有更低的复杂度和更多的可攻击轮数。
国家自然科学基金（61370194）
国家自然科学基金（61502048）
北京邮电大学网络空间安全学院，北京 100876,北京邮电大学网络空间安全学院，北京 100876
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