Many-body localization (MBL) describes a quantum phase where an isolated interacting system subject to sufficient disorder displays non-ergodic behaviour, evading thermal equilibrium that occurs under its own dynamics. Previously, the thermalization–MBL transition has been largely characterized with the growth of disorder. Here, we explore a new axis, reporting on an energy-resolved MBL transition using a 19-qubit programmable superconducting processor, which enables precise control and flexibility of both disorder strength and initial state preparation. We observe that the onset of localization occurs at different disorder strengths, with distinguishable energy scales, by measuring time-evolved observables and quantities related to many-body wave functions. Our results open avenues for the experimental exploration of many-body mobility edges in MBL systems, whose existence is widely debated due to the finiteness of the system size, and where exact simulations in classical computers become unfeasible.