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期刊论文

Effect of steady and oscillatory shear stress on F-actin content and distribution in neutrophils

陈槐卿Huai-Qing Chen a Wei Tian a Yun-Shuang Chen a Liang Li a Jeffrey Raum b and K.-L. Paul Sung bc*

Biorheology 41(2004)655-664,-0001,():

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摘要/描述

We investigated neutrophil activation, specifically F-actin content and distribution, in situations mimicking the in vivo environment using steady and oscillatory shear. Under low steady shear (<150 s−1) F-actin levels were decreased for both treated (n-formyl-L-methioryl-L-leucyl-L-phenylalanine (fMLP)) and untreated neutrophils. The F-actin content increased with a change to higher steady shear levels. Neutrophils show the same behavior of decreased F-actin content for oscillatory shear (26.7 s−1) as they did for steady shear. In both situations, the low shear levels caused a decrease in F-actin content. However, as the magnitude of the shear rate increased, cells showed a reversal to increasing F-actin content. Shear caused a decrease in F-actin in the cell cortex for both control and fMLP treated cells. Ctyochalasin B (CB), a common F-actin assembly blocker, significantly decreased F-actin content. The results indicate that neutrophils regulate their actin network based on the level and type of shear stress they encounter in the bloodstream.

版权说明:以下全部内容由陈槐卿上传于   2005年07月20日 19时47分31秒,版权归本人所有。

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