Nucleotide Binding to Nucleoside Diphosphate Kinases: X-ray Structure of Human NDPK-A in Complex with ADP and Comparison to Protein Kinases，成果详细信息-中国科技论文在线

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Nucleotide Binding to Nucleoside Diphosphate Kinases: X-ray Structure of Human NDPK-A in Complex with ADP and Comparison to Protein Kinases

陈宇星， Yuxing Chen ， Sarah Gallois-Montbrun ， Benoit Schneider ， Michel V

J. Mol. Biol. (2003) 332, 915-926，-0001，（）：

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摘要/描述

NDPK-A, product of the nm23-H1 gene, is one of the two major isoforms of human nucleoside diphosphate kinase. We analyzed the binding of its nucleotide substrates by fluorometric methods. The binding of nucleoside triphosphate (NTP) substrates was detected by following changes of the intrinsic fluorescence of the H118G/F60W variant, a mutant protein engineered for that purpose. Nucleoside diphosphate (NDP) substrate binding was measured by competition with a fluorescent derivative of ADP, following the fluorescence anisotropy of the derivative. We also determined an X-ray structure at 2.0

关键词: nm23 ， nucleotide conformation ， fluorescence anisotropy ， reverse author trans ， c ， r ， i ， p ， t ， ase inhibitors

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