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期刊论文

Kinetic model of lysozyme renaturation with the molecular chaperone GroEL

董晓燕Xiao-Yan Dong Yu-Bing Wang Xiao-Guang Liu & Yan Sun*

Biotechnology Letters 23: 1165-1169, 2001.,-0001,():

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摘要/描述

From the renaturation kinetics of denatured/reduced lysozyme assisted by the molecular chaperone GroEL, a simplified kinetic model was established based on the competition between protein folding and aggregation. In the presence of GroEL and ATP, the aggregate formation was a second order reaction. With 2mM ATP, a renaturation yield of 90% at a high renaturation rate was obtained when the molar ratio of GroEL to lysozyme was 1: 1.

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