Summary-Ras-VEGF concerned angiogenesis is correlated with oncogene maintenance, tumori-gagesis, metastasis and resistance to anti cancer therapies; however, this association is not clearly elucidated by serum VEGF, due to VEGF signalling in blood cells themselves The present study aimed to elucidate tumorigenic VEGF eignalling in eight human HCC cell types and reveal the kinetics of tumorigenic VEG F signalling in three time intervals, thereby discovering the relationships Of VEGF concemed argiogenesis signalling with the extent of the human HCC cell growth, metastasis and resistance to anti-cancer drugs, by using the poorly metastatic SMMC7721, 7402/D+ (doxorubicin- resistance) and 7402/b (dOxoruhicin withdrawal), the highly metastatic MHCC1 non-transfected human HCC cell lines, and the highly melaetatic A3-1, FS, Ftf and E3 human HCC cell lines trans teated with exgressing green fluorescence protein into the phenotype of MHCC1 ceils, and quantita- tive 'sandwtch" ELISA analyses. The unique results indicated attdbetes and objective taws as follows Human HCC cell growth requites time dependent tumorigenic VEGF signalling; levets ot VEGF sig nailing are positively correlated with each cell phenotyge itself; and levels at VEGF signalling are inversely correlated with the possibility of rnetaslaSis and drug resistance. The contrast data first feveal important elues for exploring dual metastatic mechanisms via tumor celt-generated non- endothelium vasculogenesis and VEGF-endetheliurn-attached anglogeneeis that may be essential for developing novel strategies aimed at VEGF-concerned signal networks in iSchemic/metastatic dis- eases arid transgenic models.